Improved protein identification through the use of unstained gels |
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| Authors: | Loiselle David R Thelin William R Parker Carol E Dicheva Nedyalka N Kesner Barry A Mocanu Viorel Wang Frank Milgram Sharon L Warren Maria R Esteban Borchers Christoph H |
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| Institution: | Department of Biochemistry & Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA. |
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| Abstract: | In this work, a method for improved protein identification of low-abundance proteins using unstained gels, in combination with robotics and matrix-assisted laser desorption/ionization tandem mass spectrometry, has been developed and evaluated. Omitting the silver-staining process resulted in increased protein identification scores, an increase in the number of peptides observed in the MALDI mass spectrum, and improved quality of the tandem mass spectrometry data. |
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