Efficiency of translation initiation by non-AUG codons in Saccharomyces cerevisiae. |
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| Authors: | J M Clements T M Laz and F Sherman |
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| Institution: | Department of Biochemistry, University of Rochester School of Medicine and Dentistry, New York 14642. |
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| Abstract: | The quantitative levels of initiation of protein synthesis at codons other than AUG were determined with a CYC7-lacZ fused gene in the yeast Saccharomyces cerevisiae. AUG was the only codon which efficiently initiated translation, although some non-AUG codons allowed initiation at very low efficiency, below 1% of the normal level. Since translation initiates at codons other than AUG in at least two wild-type genes from eucaryotes, other factors presumably play a role in enhancing the activity of non-AUG codons. |
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