Thermal performance of quartz capillaries for vitrification |
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Authors: | Risco Ramon Elmoazzen Heidi Doughty Marshal He Xiaoming Toner Mehmet |
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Institution: | aThe Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, 51 Blossom Street, Boston, MA 02114, USA;bEscuela Superior de Ingenieros, Universidad de Sevilla, 41092 Sevilla, Spain |
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Abstract: | In this paper we report the thermal behavior of a new approach for vitrification. Thermal performance of traditional open pulled straws is compared with a new technique based on the combined use of quartz capillaries with slush nitrogen. This new method of vitrification achieved ultrafast cooling rates of 250,000 °C/min. As a result, a much lower concentration of cryoprotectant was needed to reach vitrification. In fact, a cryoprotectant solution typically used in oocyte slow freezing protocols was shown to remain transparent after cooling to liquid nitrogen temperatures indicating apparent “vitrification”. This approach offers a new and very promising technique for vitrification of cells using low levels of cryoprotectants. |
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Keywords: | Vitrification 1 2-Propanediol Sucrose Quartz microcapillary Open pulled straw Slush nitrogen Oocyte Ultrafast cooling rates |
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