A new vitrification device that absorbs excess vitrification solution adaptable to a closed system for the cryopreservation of mouse embryos |
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| Institution: | 1. School of Veterinary Medicine, Kitasato University, Aomori, 034-8628, Japan;2. Kyoto R&D Laboratory, Mitsubishi Paper Mills Limited, Kyoto, 617-8666, Japan;3. Department of Obstetrics and Gynecology, Faculty of Medicine, Tokyo University, Tokyo, 113-8655, Japan;1. Department of Biomechatronics Engineering, National Pingtung University of Science and Technology, Taiwan, ROC;2. Department of Vehicle Engineering, National Pingtung University of Science and Technology, Taiwan, ROC;3. Industrial Technology Research Institute, Taiwan, ROC;4. Advanced Institute of Manufacturing with High-Tech Innovations, Department of Mechanical Engineering, National Chung Cheng University, Taiwan, ROC;1. Department of Surgery, University of Alberta, Edmonton, Alberta, T6G 2B7, Canada;2. Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, T6G 1H9, Canada;3. Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, T6G 2R7, Canada;4. Department of Orthopaedic Surgery, First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, 515300, China;1. Camel Reproduction Centre, Dubai, United Arab Emirates;2. School of Science and Technology, University of New England, Armidale, New South Wales, Australia;1. Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran;2. Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran;1. IVI Valencia, IVI-RMA Global, Plaza Policía Local, 3, 46015, Valencia, Spain;2. Embryotools, Parc Científic de Barcelona, Av. Doctor Marañon, 8, 08028, Barcelona, Spain;1. Fraunhofer Institute for Biomedical Engineering, 66280, Sulzbach, Germany;2. Department of Molecular and Cellular Biotechnology, Saarland University, 66123, Saarbrücken, Germany;3. Faculty of Marine Science, Universidad Católica del Norte, Coquimbo, Chile |
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| Abstract: | Several closed vitrification devices that avoid contact with liquid nitrogen have been reported. Recently, based on the Kitasato Vitrification System (KVS), we developed the Closed-KVS, which is a closed vitrification device. The KVS is an open vitrification device that can absorb excess vitrification solution. In this study, we performed two experiments to evaluate the efficacy of the Closed-KVS as a vitrification device for the cryopreservation of mouse embryos at the blastocyst and two-cell stage. In the first experiment, the blastocysts were vitrified using either the Closed-KVS or the KVS (control device). The survival, re-expansion, and hatching rates were not significantly different between embryos vitrified using the Closed-KVS and those vitrified using the KVS. In the second experiment, we evaluated the embryonic development of the two-cell stage embryos vitrified using the Closed-KVS. There were no significant differences in the survival, blastocyst formation, or hatching rates between vitrified or non-vitrified embryos. Additionally, we evaluated the cooling and warming rates of these devices using a numerical simulation method. The cooling rates of the Closed-KVS were similar regardless of whether the outer cap was pre-cooled and were lower than those of the KVS. However, the warming rates of the Closed-KVS (irrespective of cap pre-cooling) were the same as those of the KVS (612,000 °C/min). In summary, the Closed-KVS is a novel closed vitrification device for the cryopreservation of mouse embryos at the blastocyst and two-cell stage. |
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| Keywords: | Mouse Embryos Vitrification Closed system KVS Cryopreservation KVS"} {"#name":"keyword" "$":{"id":"kwrd0045"} "$$":[{"#name":"text" "_":"Kitasato Vitrification System |
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