Toxicity tests of cryoprotecting agents for Mytilus galloprovincialis (Lamark, 1819) early developmental stages |
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| Institution: | 1. Marine Biological Resources Functional Preservation Service, Estación de Ciencias Mariñas de Toralla, Universidade de Vigo, Illa de Toralla, 36331, Coruxo, Vigo, Spain;2. Departamento de Ecología y Biología Animal, Facultad de Ciencias del Mar, Universidad de Vigo, Vigo, Spain;1. Fundamental and Applied Cryobiology Group, Biochemistry Cellular and Molecular Biology, University of Tennessee, 37912, Knoxville, TN, United States;2. Marine Biological Resources Functional Preservation Service, Estación de Ciencias Mariñas de Toralla, Universidade de Vigo, Illa de Toralla, 36331, Coruxo, Vigo, Spain;1. Ifremer, UMR 6539, PFOM Department, Station Expérimentale d’Argenton, Argenton, France;2. IUEM, UMR 6539, Lemar, Technopole Brest Iroise, Plouzané, France;3. Ifremer, UMR 6539, PFOM Department, Technopole Brest-Iroise, Plouzané, France;4. Ifremer, UMR 248, Marbec, Station Ifremer, Palavas, France;1. CCMAR, University of Algarve, Campus of Gambelas, 8005-139 Faro, Portugal;2. IPMA, IP, Av.5 de Outubro s/n, 8700-305 Olhão, Portugal;3. IFREMER, UMR 6539 LEMAR, Departement PFOM/Laboratoire ARN Station Expérimentale d’Argenton, Presqu’île du Vivier, 29840 Argenton, France |
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| Abstract: | Global aquaculture production of blue mussel has increased over last years. This work reaffirms the great potential of cryopreservation technique on mussel industry and overcome economic barriers a cause of a traditional and rudimentary management and continue growing. The aim of this work is to set some preliminary basis attending to toxicity of cryoprotecting agents (CPAs) on different development stages of Mytilus galloprovincialis as a start point to develop a stable cryopreservation protocol. Toxicity tests were carried out by using common CPAs (dimethyl-sulfoxide (Me2SO), glycerol, (GLY), propylene glycol (PG) and ethylene glycol (EG)) in a range from 0.5 to 3 M on fertilized egg, trochophore larva, and D-larva of Mytilus galloprovincialis. Results evidenced more resistance of older development stages to toxicity. Of all CPAs tested, toxicity testing highlights PG or EG as suitable CPAs for cryopreservation of early development stages; whereas D-larva was unaffected by any of the CPAs tested. Preliminary cryopreservation trials were developed to obtain information into cell cryoprotection. Further research should be focused on membrane permeability and other parameters, such as the balance between toxicity and cryoprotective effect of CPAs. |
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| Keywords: | Mussel Cryopreservation Cryoprotecting agents Marine invertebrates Toxicity |
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