Effect of polyvinyl alcohol on survival and function of angora buck spermatozoa following cryopreservation |
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| Institution: | 1. Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna, Km 16, Salobrinho, Ilhéus, BA 45650-000, Brazil;2. Departamento de Biologia Geral, Universidade Federal de Viçosa, Av. Peter Henry Rolfs s/n, Campus Universitário, Viçosa, MG 36570-000, Brazil;3. Departamento de Medicina Veterinária, Pontifícia Universidade Católica de Minas Gerais, Unidade Betim, Rua do Rosário, 1081, Angola, Betim, MG 32604-115, Brazil;4. Laboratório de Imunologia, Fundação Ezequiel Dias - FUNED, Rua Conde Pereira Carneiro 80, Gameleira, Belo Horizonte, MG 30510-010, Brazil;5. Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Rua Leonil Prata 819, Alípio de Melo, Belo Horizonte, MG 30840780, Brazil;1. BAAC, Aquatic Environments Continental Biology, Institute of Biological Sciences, Federal University of Rio Grande, Rio Grande, RS, Brazil;2. RAC – Compared Animal Reproduction, Institute of Biological Sciences, Federal University of Rio Grande, Rio Grande, RS, Brazil;3. ReproPel, Faculty of Veterinary Medicine, Campus Capon Lion, Federal University of Pelotas, Pelotas, Brazil;4. Physiological Sciences, Comparative Animal Physiology, Institute of Biological Sciences, Federal University of Rio Grande, Rio Grande, RS, Brazil;5. College Animal Science, Campus Capon Lion, Federal University of Pelotas, Pelotas, Brazil;1. Department of Biochemistry and Molecular and Cellular Biology-Institute of Research in Environmental Sciences of Aragon (IUCA), University of Zaragoza, E-50013 Zaragoza, Spain;2. Department of Animal Medicine and Surgery, Autonomous University of Barcelona, E-08193, Cerdanyola Del Vallès, Spain;3. Department of Animal Health and Anatomy, Autonomous University of Barcelona, E-08193, Cerdanyola Del Vallès, Spain;4. Department of Animal Selection and Reproduction, The Regional Agri-Food Research and Development Service of Asturias (SERIDA), E-33394 Gijón, Spain;5. Department of Animal Pathology, University of Santiago De Compostela, E-15705 Lugo, Spain;1. Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Department of Animal Science, Faculty of Agriculture Science, Lorestan University, Khorramabad, Iran;3. Research Center for New Technology in Life Science Engineering, University of Tehran, Iran;4. Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran;5. Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modarres University, Tehran, Iran |
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| Abstract: | The aim of this study was to examine effects of polyvinyl alcohol (PVA) on buck semen quality. Seventy-five ejaculates were collected and diluted in Tris-egg yolk extender containing one of three PVA co-polymers of 9, 18 and 100 kDa. Five different concentrations 0.001, 0.01, 0.1, 1 and 2% of the PVA co-polymers were added to the extender with respected to the decreasing glycerol concentrations of 5, 4, 3, 3, 2% respectively. Following freeze-thaw, sperm motility, viability, acrosome-intact spermatozoa and mitochondrial membrane potentials were analysed. During freezing, sperm seeding temperature were recorded with a cryo-thermometer. PVA 2% glycerol group gained 8.2 ± 1 °C latent heat plateau difference comparing to control. Highest motility was found in PVA 18 kDa with regardless of the dosage (P < 0.001). All PVA copolymers gained higher motility independently in all other dosage groups (except PVA 2%) comparing to control (P < 0.001). Live spermatozoa rate between treatment groups were statistically insignificant (P = 0.953), however, when moribund sperm were gated out PVA 9 induced better protection with respect to other groups (P < 0.05). Intact acrosome rate was statically higher in PVA groups (P < 0.002) and subgroups (P < 0.001). Mitochondrial membrane potential was higher in all experimental groups comparing to control group (P < 0.001). PVA co-polymer concentrations of 0.01, 0.1, 1 and 2% v/v (PBS: PVA) decreased the concentration of glycerol required for freezing in a 100 ml volume by 0, 1, 2, 2, and 3% v/v from the control dose (5%), respectively. In conclusion, synthetic PVA-derived ice blocking agents offer new opportunities for improving the post-thaw buck sperm quality. |
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| Keywords: | Angora goat CASA Cytoreader Cryopreservation Polyvinyl alcohol Seeding Viability |
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