Vitrification of in vitro produced bovine embryos: Effect of embryonic block and developmental kinetics |
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Authors: | V Asgari SM Hosseini M Forouzanfar M Hajian MH Nasr-Esfahani |
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Institution: | 1. Department of Reproduction and Development, Reproductive Biomedicine Centre, Royan Institute of Biotechnology, ACECR, Isfahan, Iran;2. Department of Basic Science, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran;3. Department of Embryology, Reproductive Biomedicine Centre, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran |
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Abstract: | In order to investigate whether the kinetics and stage of embryo development affect cryosurvival of in vitro produced bovine embryos, cleaved embryos were categorized in six groups based on their developmental kinetics regarding the stage of embryonic block in bovine (8–16 cell stage): I and II – early (day 2) and late (day 3) 5–8 cell, III and IV – early (day 3) and late (day 4) 8–16 cell, and V and VI – early (day 4) and late (day 5) morula. The cryosurvival and developmental competence of these embryos were compared with each other and also with the corresponding control groups. The potential of 5–8 cell stage embryos to survive vitrification and further develop towards blastocyst stage was significantly lower than vitrified and un-vitrified 8–16 cell and morula stage embryos. These results suggest that, the survival rate and potential of embryos to develop towards blastocyst stage might be affected by the kinetic of the embryo development. Moreover, the results of this study indicated that the optimal stages of early embryo vitrification are post-embryonic block. |
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Keywords: | EGA embryonic genome activation COC cumulus oocyte complex VS vitrification solution Me2SO dimethyl sulfoxide EG ethylene glycol RT room temperature ES equilibration solution TCN total cell number ICM inner cell mass TE trophectoderm |
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