Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa |
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| Institution: | 1. Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa 16059, Turkey;2. Department of Biostatistics, Faculty of Veterinary Medicine, Uludag University, Gorukle/Bursa 16059, Turkey;3. Department of Pharmacology, Faculty of Medicine, Uludag University, Gorukle/Bursa 16059, Turkey;1. Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran;3. Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran;1. Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle, Département des Sciences Animales, 2425, rue de l’Agriculture, Université Laval, Québec, QC, G1V 0A6, Canada;2. Département d''Obstétrique et de Gynécologie, Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle, Université Laval, Axe reproduction, santé périnatale et santé de l''enfant, Centre de recherche du CHUQ-CHUL, Québec, G1V 0A6, Canada;1. Animal Reproduction Laboratory, Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan;2. Reproductive Physiology Laboratory, Department of Animal Sciences, Quaid–i–Azam University, Islamabad, Pakistan;1. Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura 281001, Uttar Pradesh, India;2. Department of Veterinary Medicine, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura 281001, Uttar Pradesh, India;1. College of Biotechnology, U.P. Pandit Deendayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India;2. Department of Veterinary Physiology, U.P. Pandit Deendayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India;3. Department of Biochemistry, U.P. Pandit Deendayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India;4. College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan, Mathura, 281001, Uttar Pradesh, India;1. Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran;2. Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;3. Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran |
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| Abstract: | The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6 h incubation when the extender was supplemented by 5 mM BHT or cysteamine. |
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| Keywords: | Freeze-drying Goat semen Cryopreservation Antioxidants Incubation resilience |
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