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The effect of cryogenic storage on human erythrocyte membrane proteins as determined by polyacrylamide-gel electrophoresis |
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| Authors: | EM Skrabut JP Crowley N Catsimpoolas CR Valeri |
| Institution: | 21. Department of Hematology, Boston University Medical Center, Boston, Massachusetts 02118, U.S.A.;22. Biophysics Laboratory, Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 U.S.A.;3. Department of Medicine, Harvard Medical School, Peter Bent Brigham Hospital, Boston, Massachusetts 02115 U.S.A.;4. Biophysics Laboratory, Department of Nutrition and Food Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 U.S.A.;5. Naval Blood Research Laboratory, Chelsea, Massachusetts 02150 U.S.A. |
| Abstract: | A study was conducted to determine the effects of freezing on the major membrane proteins of isolated human erythrocyte membranes. Membranes in low or normal ionic strength medium were frozen at slow or fast freezing rates. The membrane protein composition and elution of proteins from the membranes were studied utilizing polyacrylamide-gel electrophoresis in a sodium dodecyl sulfate or an acetic acid-urea-phenol solvent system. Neither a change in the composition of the membrane proteins nor any elution of membrane protein during freezing and thawing was observed. The data indicate that any human erythrocyte membrane damage during freezing and thawing was not related to a change in major membrane protein composition. Human red cell membranes were stable at ?80 or ?196 °C in the absence of a cryoprotective agent. |
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