Evaluation of the Cryotech Vitrification Kit for bovine embryos |
| |
Authors: | C Gutnisky GM AlvarezPD Cetica GC Dalvit |
| |
Institution: | Area of Biochemistry, School of Veterinary Sciences, University of Buenos Aires, Argentina |
| |
Abstract: | The purpose of this work was to assess commercially available Cryotech Vitrification Kit, in terms of survival, in vitro development and pregnancy rate for bovine embryos. Cumulus-oocyte complexes (COCs) were recovered from ovaries obtained from slaughtered cows and then matured in vitro for 22 h. COCs were fertilized by sex-sorted sperm in IVF-mSOF and cultured in IVC-mSOF for 7 days to the blastocyst stage. Blastocysts were vitrified with the Cryotech Vitrification Kit® and then either warmed to check viability or transferred to synchronized heifers. We observed 100% survival of the in vitro produced blastocysts and obtained the same pregnancy rate (46.8%) as that obtained using fresh in vitro produced blastocysts. We thus conclude that the Cryotech vitrification method is a valid alternative to other vitrification or slow-cooling methods in the bovine species and that it is ready for livestock production. |
| |
Keywords: | Bovine embryo vitrification Bovine embryo IVP Cryotech Vitrification |
本文献已被 ScienceDirect 等数据库收录! |