| 新的产弹性蛋白酶菌株的筛选与鉴定 |
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| 引用本文： | 刘双发,安德荣,苟丽霞,李 娜.新的产弹性蛋白酶菌株的筛选与鉴定[J].微生物学通报,2009,36(9):1345-1349. |
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| 作者姓名： | 刘双发 安德荣 苟丽霞 李 娜 |
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| 作者单位： | 西北农林科技大学植保学院与陕西省农业分子生物学重点实验室,陕西,杨凌,712100 |
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| 基金项目： | 国家863计划项目(No. 2007AA021503); 教育部长江学者和创新团队发展计划资助(No. 200558) |
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| 摘 要： | 本研究从秸秆中分离得到一株弹性蛋白酶高产菌, 并对该菌株进行鉴定, 以期为实现其工业化生产提供理论依据。采用酪蛋白(脱脂奶粉)进行初筛后, 以弹性蛋白(牛筋)进行复筛, 并对筛选结果进行检验, 然后对所得菌株结合形态、生理生化特性和16S rRNA基因序列进行分类鉴定, 得到一株弹性蛋白酶高产菌株LSF-97。结果显示, 菌株LSF-97与短小芽孢杆菌同源率达到100%, 形态及生理生化特征与模式菌也显示高度一致性, 故将其确定为短小芽孢杆菌。
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| 关 键 词： | 芽孢杆菌 蛋白酶 筛选 鉴定 |
Screening and Identification of a New Elastase-producing Strain |
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| LIU Shuang-F,AN De-Rong,GOU Li-Xia and LI Na.Screening and Identification of a New Elastase-producing Strain[J].Microbiology,2009,36(9):1345-1349. |
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| Authors: | LIU Shuang-F AN De-Rong GOU Li-Xia and LI Na |
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| Institution: | College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China;College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China;College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China;College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling, Shaanxi 712100, China |
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| Abstract: | The study provide a theoretical basis for the industrial production of a elastase-high-yield strain which was isolated from the straw, and the selected strain was identified. The screening strategy included casein (skim milk) plate selecting and elastin (beef tendon) re-screening. And then, the morphological, physiological and biochemical characteristics as well as 16S rRNA sequence homology of the selected strain were studied. Finally, the strain LSF-97 which had a excellent decomposing ability to beef tendon was obtained. The results showed that the strain LSF-97 is relative to the Bacillus pumilus with 100 % similar in sequence under the phylogenetic tree, the morphological and physiological and biochemical characteristics are also consistent with pattern of bacteria. So it was identified as Bacillus pumilus. |
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| Keywords: | Bacillus Elastase Screening Identification |
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