| 3种淡水环境中低核酸含量细菌的滤过性 |
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| 引用本文： | 刘杰,宋宇昊,赵志鹏,王莹莹.3种淡水环境中低核酸含量细菌的滤过性[J].微生物学通报,2016,43(9):1895-1901. |
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| 作者姓名： | 刘杰 宋宇昊 赵志鹏 王莹莹 |
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| 作者单位： | 南开大学环境科学与工程学院 环境污染过程与基准教育部重点实验室 天津市城市生态环境修复与污染防治重点实验室 天津 300071,南开大学环境科学与工程学院 环境污染过程与基准教育部重点实验室 天津市城市生态环境修复与污染防治重点实验室 天津 300071,南开大学环境科学与工程学院 环境污染过程与基准教育部重点实验室 天津市城市生态环境修复与污染防治重点实验室 天津 300071,南开大学环境科学与工程学院 环境污染过程与基准教育部重点实验室 天津市城市生态环境修复与污染防治重点实验室 天津 300071 |
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| 基金项目： | 国家自然科学基金项目(No. 31270545) |
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| 摘 要： | 【目的】增加低核酸含量(LNA)细菌与过滤性细菌之间的认识。【方法】采用流式细胞技术(FCM)、变性梯度凝胶电泳(DGGE)及统计学分析研究3种典型淡水环境中细菌群落与滤过性。【结果】LNA细菌与过滤性细菌在数量上具有很好的相关性(y=0.646x+22.42,R2=0.984,P0.01),细菌的滤过性不仅与细菌大小有关,还与细胞整体形状及其伸缩性有关;细菌群落组织与LNA细菌比重呈负相关性,而与HNA细菌呈正相关性。【结论】0.45μm膜过滤对水样微生物群落中的LNA细菌具有极强的筛选效果;细菌群落组织与其基于流式细胞技术测定的基因含量具有密切联系。
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| 关 键 词： | 流式细胞技术(FCM)，低核酸含量(LNA)细菌，高核酸含量(HNA)细菌，过滤性细菌，变性梯度凝胶电泳(DGGE)，群落组织 |
Filterability of bacteria with low nucleic acid content in three freshwater environments |
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| LIU Jie,SONG Yu-Hao,ZHAO Zhi-Peng and WANG Ying-Ying.Filterability of bacteria with low nucleic acid content in three freshwater environments[J].Microbiology,2016,43(9):1895-1901. |
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| Authors: | LIU Jie SONG Yu-Hao ZHAO Zhi-Peng and WANG Ying-Ying |
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| Institution: | College of Environmental Science and Engineering, Nankai University, Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Tianjin 300071, China,College of Environmental Science and Engineering, Nankai University, Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Tianjin 300071, China,College of Environmental Science and Engineering, Nankai University, Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Tianjin 300071, China and College of Environmental Science and Engineering, Nankai University, Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Tianjin 300071, China |
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| Abstract: | |
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| Keywords: | Flow cytometry (FCM) Low nucleic acid content (LNA) bacteria High nucleic acid content (HNA) bacteria Filterable bacteria Denaturing gradient gel electrophoresis (DGGE) Community organization |
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