| 一株生物表面活性剂产生菌的分离及其特性研究 |
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| 引用本文： | 孙 燕,洪 青,李顺鹏.一株生物表面活性剂产生菌的分离及其特性研究[J].微生物学通报,2009,36(8):1110-1116. |
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| 作者姓名： | 孙 燕 洪 青 李顺鹏 |
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| 作者单位： | 南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 国家863计划项目(No. 2007AA061101); 科技部自然科技资源平台项目(No. 2005DKA21201-2) |
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| 摘 要： | 模炼油厂污泥中分离得到1株生物表面活性剂产生菌C-3, 根据其生理生化特性和16S rDNA序列相似性分析, 将其鉴定为铜绿假单胞菌(Pseudomonas aeruginosa)。初步研究了其产生物表面活性剂的最适条件, 在以植物油为碳源、30°C、初始pH 8、Ca2+浓度20 mg/L、250 mL三角瓶中装75 mL发酵液的条件下, 最利于菌株的生长和生物表面活性剂产生。它的成分为糖脂类物质, 临界胶束浓度(CMC)为50 mg/L, 具有很好的增溶效果。
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| 关 键 词： | 生物表面活性剂 分离 最适条件 成分分析 |
Isolation and Characterization of a Biosurfactant-producing Bacterium |
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| SUN Yan,HONG Qing and LI Shun-Peng.Isolation and Characterization of a Biosurfactant-producing Bacterium[J].Microbiology,2009,36(8):1110-1116. |
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| Authors: | SUN Yan HONG Qing and LI Shun-Peng |
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| Institution: | Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China;Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China |
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| Abstract: | A biosurfactant producing bacterial strain C-3 was isolated from the sludge of a petroleum refinery and identified as Pseudomonas aeruginosa. The optimal conditions for the growth and biosurfacant production of strain C-3 were investigated. The results showed that strain C-3 could grow well and produce the largest amount of biosurfacant under the following conditions: seed-oil as sole carbon source, 30°C, initial pH 8, Ca2+ concentration of 20 mg/L, 75 mL medium in 250 mL Erlenmeyer flask. The biosurfactants produced by strain C-3 was identified as lipopeptides, showing high surface activity and emulsifying efficiency with critical micelle concentration (CMC) being 50 mg/L. |
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| Keywords: | Biosurfactant Isolation Optimal conditions Biosurfactant analysis |
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