东方蜜蜂微孢子虫侵染意大利蜜蜂工蜂过程的nce-miR-23928及其靶基因表达谱

【背景】东方蜜蜂微孢子虫(Nosema ceranae)专性侵染成年蜜蜂中肠上皮细胞而导致的微孢子虫病给养蜂业造成严重损失。【目的】检测东方蜜蜂微孢子虫nce-miR-23928及其靶基因在侵染意大利蜜蜂(Apis mellifera ligustica)工蜂过程的表达谱，为深入探究nce-miR-23928在东方蜜蜂微孢子虫侵染中的功能及调控机制提供依据。【方法】通过RNAhybrid、miRanda和TargetScan软件预测nce-miR-23928的靶基因。使用BLAST工具将上述靶基因比对到基因本体论(geneontology,GO)、京都基因和基因组百科全书(Kyoto encyclopedia of genes and genomes, KEGG)、Nr和Swiss-Prot数据库以获得相应注释。采用实时荧光定量PCR(realtimequantitativePCR,RT-qPCR)技术检测nce-miR-23928及其靶基因在东方蜜蜂微孢子虫侵染意蜂工蜂过程中的相对表达量。【结果】相较于接种后1 d (1 day post infection, 1 dpi),nce-...

Expression profiles of nce-miR-23928 and its target genes in the Nosema ceranae infection of Apis mellifera ligustica workers

Background] Nosema ceranae exclusively infects midgut epithelial cells of adult bees, and the resulting microsporidiosis causes severe losses to the beekeeping industry. Objective] This study aimed to determine the expression profiles of nce-miR-23928 and its target genes during the N. ceranae infection of Apis mellifera ligustica workers, and to provide basis for further investigation on the function and regulatory mechanism of nce-miR-23928 during the infection. Methods] Target genes of nce-mir-23928 were predicted by RNAhybrid, miRanda and TargetScan. Blast was used to perform annotation of the aforementioned target genes in gene ontology (GO), kyoto encyclopedia of genes and genomes (KEGG), Nr and Swissprot. Real time quantitative PCR (RT-qPCR) was employed to detect relative expression of nce-miR-23928 and its target genes. Results] As compared with the condition at 1 day post infection (1 dpi), the expression of nce-miR-23928 remained unchanged at 2 dpi, but was all down-regulated at 4 6, 8 dpi (P <0.05), presenting an overall reduced expression trend. Additionally, 15 target genes of nce-miR-23928 were predicted, among which 9, 4, 15 and 9 were annotated in GO (3 items), KEGG (7 pathways), Nr and Swiss-prot, respectively. As compared with the condition at 1 dpi, the expression of target gene ABCT was significantly down-regulated at 2, 4, 6, 8 dpi, while the expression of target gene STPK was significantly up-regulated at 4, 6, 8 dpi, displaying an overall elevation trend. Conclusion] These results illuminated the dynamic expression rules of nce-miR-23928 and its target genes ABCT and SPTK in the N. ceranae infection of A. mellifera ligustica workers, and unraveled that nce-miR-23928 putatively modulated the infection process through positively regulating the expression of ABCT and negatively regulating the expression of STPK.