兰茂牛肝菌子实体发育的关联物质及其主要通路分析

【背景】兰茂牛肝菌(Lanmaoaasiatica)等外生菌根真菌的子实体形成和发育机制仍然未知。【目的】揭示调控子实体发育的关联物质。【方法】同时运用核磁共振、气相质谱和液相质谱3种代谢组学技术，分析兰茂牛肝菌纯培养8d原基(Y8)与野生子实体(Z0)的小分子物质。【结果】Y8及Z0分别共指认出451、473种化合物；Y8vs.Z0，有362种显著或极显著上调(206种)及下调(156种)差异物质，其涉及47条调控通路。【结论】推测通过9条主要通路完成物质的深度转化及调控，极显著上调及下调差异物质如牛肝菌素可能对子实体的发育起着一定的调控作用，3种方法互相补充扩大了检测的泛度及灵敏度，这为探究兰茂牛肝菌子实体发育机理及人工培养提供了一定的理论参考。

Active components and main pathways involved in fruiting body development of Lanmaoa asiatica

Background] The fruiting body formation and development of ectomycorrhizal fungi such as Lanmaoa asiatica still unknown. Objective] To identify the active components regulating the fruiting body development. Methods] We employed Three metabolomics techniques including nuclear magnetic resonance, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry were employed to analyze the small molecular components in L. asiatica primordia cultured for 8 days (Y8) and feral sterile fruiting bodies (Z0). Results] We found that each of the three analytical methods identified 451 and 473 compounds in Y8 and Z0, respectively. A total of 362 significantly differential components were identified, including 206 up-regulated components and 156 down-regulated components, which were involved in 47 pathways. Conclusion] We hypothesized that the deep transformation and regulation of components were accomplished via nine main pathways. The significantly up-regulated and down-regulated differential components such as bolegrevilol may play a regulatory role in the development of fruiting bodies. The three methods used in this study complemented each other, expanding the scope and improving the sensitivity of the detection. The results provided some theoretical reference for deciphering the mechanism of fruiting body development and exploring the artificial cultivation of L. asiatica.