嗜水气单胞菌RB5-M1的分离鉴定及脱色条件研究

【目的】筛选具有高效脱色活性黑5能力的菌株。【方法】利用梯度浓度驯化法,从上海松江污水处理厂的活性污泥中分离获得了一株具有良好脱色能力的细菌RB5-M1。【结果】经形态学观察、生理生化特性鉴定以及16S rRNA基因序列分析,发现该菌株与嗜水气单胞菌(Aeromonas hydrophila)的同源性达到99.86%。【结论】菌株RB5-M1在35°C、pH 8.0的条件下,厌氧条件培养(氮气85%、二氧化碳6%)24 h,测定的平均脱色率可达94.1%,最高脱色率为99.8%。

Isolation, identification and biological decoloring conditions of Aeromonas hydrophila strain RB5-M1

Objective] The purpose was to develop some bacterial strains that could decolorize reactive black 5 effectively. Methods] We isolated a bacterial strain which has strong decolorization ability, by the method of domestication with gradient concentrations, from activated sludge of Songjiang sewage treatment plant in Shanghai. Results] According to its morphological, physiological characteristics and the analysis of 16S rRNA gene analyses for strain identification, >99.86 % of gene sequences in isolated strain RB5-M1 were similar to Aeromonas hydrophila compared to available gene sequences in the NCBI BLAST gene bank. Conclusion] When the strain was cultivated in anaerobic cultural incubator (nitrogen 85%, carbon dioxide 6%), 35 °C, pH 8.0; it showed a decolorization rate performance of 94.1% for average value, 99.8% for maximum value.