|
|||||
|
|
| 解磷细菌PSB3的筛选及拮抗作用的研究 | |
| 引用本文： | 余贤美,王 义,沈奇宾,李炳龙,贺春萍,郑服丛.解磷细菌PSB3的筛选及拮抗作用的研究[J].微生物学通报,2008,35(9):1398-1403. |
| 作者姓名： | 余贤美 王 义 沈奇宾 李炳龙 贺春萍 郑服丛 |
| 作者单位： | 1. 中国热带农业科学院环境与植物保护研究所,儋州,571737 2. 海南大学,环境与植物保护学院,儋州,571737 3. 中国热带农业科学院环境与植物保护研究所,儋州,571737;海南大学,环境与植物保护学院,儋州,571737 |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项资助项目，国家科技支撑计划，公益性行业(农业)科研专项基金 |
| 摘 要： | 利用有机磷细菌液体培养基进行生物富集,无机磷细菌固体培养基通过平板稀释法进行分离筛选,建立了土壤解磷细菌的筛选体系.扩增菌株PSB3的16S rDNA序列,序列测定结果显示,该片段长度为1525 bp,经Blastn搜索进行序列比对,该细菌为洋葱伯克霍尔德氏工菌(Burkholderia cepacia).对该菌株与供试的12个炭疽菌和镰刀茵菌株进行室内拮抗试验,结果显示,该菌株对Fusarium solani等6个菌株有不同程度的拮抗作用. |
| 关 键 词： | 解磷细菌 生物富集 筛选体系 16S rDNA 分子鉴定 拮抗作用 |
The Screening of Phosphorus Solubilizing Bacteria PSB3 and the Study of Its Antagonism |
|
| YU Xian-Mei,WANG Yi,SHEN Qi-Bin,LI Bing-Long,HE Chun-Ping and ZHENG Fu-Cong.The Screening of Phosphorus Solubilizing Bacteria PSB3 and the Study of Its Antagonism[J].Microbiology,2008,35(9):1398-1403. | |
| Authors: | YU Xian-Mei WANG Yi SHEN Qi-Bin LI Bing-Long HE Chun-Ping and ZHENG Fu-Cong |
| Institution: | Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737;Environment and Plant Protection College, Hainan University, Danzhou 571737;Environment and Plant Protection College, Hainan University, Danzhou 571737;Environment and Plant Protection College, Hainan University, Danzhou 571737;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737;(1. Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737) (2. Environment and Plant Protection College, Hainan University, Danzhou 571737) |
| Abstract: | The screening system of phosphorus solubilizing bacteria in the soil was established by biological enriching using organic phosphobacteria liquid medium and then screening via plate dilution method using inorganic phosphobacteria solid medium. By 16S rDNA sequencing technique, the strain PSB3 with the 16S rDNA gene of 1525 bp length was identified as Burkholderia cepacia by Blastn search in NCBI database. The results of in-door antagonism experiment of PSB3 to 12 strains of Fusarium and Colletotrichum (Fusarium solani et al) showed that PSB3 had antagonism reaction against 6 of the 12 pathogens. |
| Keywords: | Phosphorus solubilizing bacteria Biological enrichment Screening system 16S rDNA Molecular identification Antagonism |
| 本文献已被 维普 万方数据 等数据库收录！ | |
| 点击此处可从《微生物学通报》浏览原始摘要信息 | |
| 点击此处可从《微生物学通报》下载免费的PDF全文 | |