中度嗜盐菌群对酸性大红GR的脱色性能研究

【目的】获得能够在高盐环境下脱色偶氮染料的高效脱色菌群,应用于印染废水的生物处理。【方法】采用在5%盐度培养基富集的方法,从印染废水的活性污泥中富集能够在5%盐度下脱色酸性大红GR的嗜盐混合菌群,利用高通量测序方法研究其群落结构,利用静置培养的方法研究其脱色性能。【结果】该菌群可以在5%盐度、静置培养下15 h内将100 mg/L的酸性大红GR几乎完全脱色,主要由Halomonas、Salinicoccus、Nitratireductor和Aequorivita等4个属组成,Halomonas是主要的脱色菌。高浓度的Na NO_3、Na_2SO_4和Na Cl抑制菌群的脱色,其中Na NO_3抑制作用最强。该菌群的最佳脱色条件是在p H 7.0、盐度5%、30°C脱色效果最好,可脱色直接耐黑G和分散深蓝S-3BG等偶氮染料,并且具有连续脱色的能力。【结论】嗜盐菌群在处理偶氮染料废水中具有良好的应用价值。

Decoloring acid scarlet GR by a moderately halophilic bacterial consortium

Objective] To obtain efficient decoloration bacterial consortium, which were able to degrade azo dyes in high salt environment and to biodegrade the dyeing wastewater. Methods] A moderately halophilic bacterial consortium degrading of acid scarlet GR was enriched from activated sludge of dyeing wastewater. The community structure of the bacterial consortium was studied by high-throughput sequencing method, and the decoloring performance was studied under static condition. Results] The moderately bacterial consortium was mainly composed by Halomonas, Salinicoccus, Nitratireductor and Aequorivita, Halomonas was the main decoloring bacteria. The degradation was inhibited by high concentration of NaCl, Na2SO4 and NaNO3, of which the strongest inhibition is NaNO3. The optimal decolorization conditions were as follows: pH 7.0, 30 °C, 5% NaCl. The moderately halophilic bacterial consortium could also degrade direct black 19 and disperse deep blue S-3BG. After continuous bleaching for 5 rounds, the degradation rate still reached over 98%. Conclusion] The moderately halophilic bacteria consortium has strong potential to treat azo dye contaminated wastewater.