| 若尔盖高原湿地不同退化阶段的土壤细菌群落多样性 |
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| 引用本文： | 唐杰,徐青锐,王立明,丁祥,汤博,吴俐莎,冯甦,孙群,杨志荣,张杰.若尔盖高原湿地不同退化阶段的土壤细菌群落多样性[J].微生物学通报,2011,38(5):677-686. |
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| 作者姓名： | 唐杰 徐青锐 王立明 丁祥 汤博 吴俐莎 冯甦 孙群 杨志荣 张杰 |
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| 作者单位： | 四川大学生命科学学院,生物资源与生态环境教育部重点实验室,四川,成都,610064 |
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| 基金项目： | 国家“十一五”科技支撑计划项目(No. 2007BAC18B02, 2007BAC18B03); 四川省应用基础项目(No. 2007J13-077) |
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| 摘 要： | 采用传统分离培养和基于16S rDNA的变性梯度凝胶电泳(Denaturing gradient gel electrophoresis, DGGE)的方法, 研究了若尔盖高原湿地不同退化阶段土壤类型(泥炭土、沼泽土、草甸土、风沙土)和土壤深度(0 cm?20 cm、20 cm?40 cm、40 cm?60 cm)中微生物的数量、细菌群落结构及其多样性。结果表明: 微生物数量总体上不仅随着土壤类型的改变而减少(泥炭土>草甸土>沼泽土>风沙土)也随着土壤深度的增加而递减(0 cm?20 cm>20 cm?4
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| 关 键 词： | 若尔盖 湿地退化 土壤细菌群落 PCR-DGGE |
Soil bacterial community diversity under different stages of degradation in zoige wetland |
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| TANG Jie,XU Qing-Rui,WANG Li-Ming,DING Xiang,TANG Bo,WU Li-Sh,FENG Su,SUN Qun,YANG Zhi-Rong and ZHANG Jie.Soil bacterial community diversity under different stages of degradation in zoige wetland[J].Microbiology,2011,38(5):677-686. |
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| Authors: | TANG Jie XU Qing-Rui WANG Li-Ming DING Xiang TANG Bo WU Li-Sh FENG Su SUN Qun YANG Zhi-Rong and ZHANG Jie |
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| Institution: | Key Laboratory of Bio-resources and Bio-environment, Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, China |
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| Abstract: | Both culture-dependent and culture-independent methods,denaturing gradient gel electropho-resis(DGGE) based on the sequence of 16S rDNA,were used to examine the microbial quantity,bacterial community structure and diversity in different soil types(Peat soil,Swamp soil,Meadow soil and Sandy soil) and soil detphs(0-20 cm,20 cm-40 cm and 40 cm-60 cm) under different stages of degradation in Zoige Wetland.Experimental results showed that total microbial quantity decreased with the soil types(Peat soil>Meadow so... |
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| Keywords: | Zoige Wetland degradation Soil bacterial community PCR-DGGE |
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