| 青海可可西里嗜碱芽胞杆菌资源调查 |
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| 引用本文： | 王小英,刘国红,刘波,阮传清,陈峥.青海可可西里嗜碱芽胞杆菌资源调查[J].微生物学通报,2017,44(8):1847-1857. |
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| 作者姓名： | 王小英 刘国红 刘波 阮传清 陈峥 |
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| 作者单位： | 1. 福州大学生物科学与工程学院 福建 福州 350116,2. 福建省农业科学院农业生物资源研究所 福建 福州 350003,2. 福建省农业科学院农业生物资源研究所 福建 福州 350003,2. 福建省农业科学院农业生物资源研究所 福建 福州 350003,2. 福建省农业科学院农业生物资源研究所 福建 福州 350003 |
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| 基金项目： | 国家自然科学基金项目(No. 31370059)；福建省自然科学基金项目(No. 2016J01129)；福建省重大专项项目农业科技重大专项项目(No. 2015NZ0003)；福建省农科院科技创新团队PI项目(No. 2016PI-43)；福建省农科院青年科技英才计划项目(No. YC2015-17)；福建省公益项目(No. 2015R1018-7，2015R1101012-9)；农业部948项目(No. 2016-X21) |
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| 摘 要： | 【目的】了解可可西里嗜碱芽胞杆菌资源多样性及产酶多样性,为芽胞杆菌功能资源挖掘和菌剂开发提供基础。【方法】采用Horikoshi I培养基,通过可培养法分离青海可可西里土壤中的嗜碱芽胞杆菌,利用16S r RNA基因序列初步鉴定分离获得的芽胞杆菌。采用透明圈法分析分离菌株的产蛋白酶、纤维素酶及木聚糖酶活性。【结果】从青海可可西里土壤中共分离获得66株嗜碱芽胞杆菌,根据16S r RNA基因序列相似性分析,发现它们隶属于6个属22个种,分别为芽胞杆菌属(Bacillus)、纤细芽胞杆菌属(Gracilibacillus)、喜盐芽胞杆菌属(Halobacillus)、咸海鲜芽胞杆菌属(Jeotgalibacillus)、类芽胞杆菌属(Paenibacillus)和嗜冷芽胞杆菌属(Psychrobacillus),其中以芽胞杆菌属(Bacillus)为优势属。2株嗜碱芽胞杆菌与它们最近匹配模式菌株的16S r RNA基因序列相似性为97.00%和98.65%,为潜在新种。三种酶活检测结果表明产酶菌株约占总分离菌株的95.00%,其中55株具有产蛋白酶活性,27株具有产纤维素酶活性,8株能够产木聚糖酶。【结论】青海可可西里蕴藏着较丰富的嗜碱芽胞杆菌资源及丰富的产酶资源,为后续嗜碱芽胞杆菌的挖掘提供理论基础。
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| 关 键 词： | 可可西里，嗜碱芽胞杆菌，蛋白酶，纤维素酶，木聚糖酶 |
Survey of alkaliphilic Bacillus-like resources in Kekexili, Qinghai |
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| WANG Xiao-Ying,LIU Guo-Hong,LIU Bo,RUAN Chuan-Qing and CHEN Zheng.Survey of alkaliphilic Bacillus-like resources in Kekexili, Qinghai[J].Microbiology,2017,44(8):1847-1857. |
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| Authors: | WANG Xiao-Ying LIU Guo-Hong LIU Bo RUAN Chuan-Qing and CHEN Zheng |
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| Institution: | 1. College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350116, China,2. Agricultural Bio-resource Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China,2. Agricultural Bio-resource Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China,2. Agricultural Bio-resource Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China and 2. Agricultural Bio-resource Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China |
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| Abstract: | Objective] To provide a foundation for new resource mining of the alkalophilic Bacillus-like species and development of microbial agents, we studied the species distribution and diversity of Bacillus-like isolated from soil samples in Kekexili of Qinghai Province. Methods] Alkalophilic Bacillus species were isolated from soil samples using the cultural method on Horikoshi I medium and preliminarily identified based on 16S rRNA gene sequences. Then, strains with ability of protease-, cellulases- and xylanase-producing activity were screened using transparent zone method. Results] A total of 66 strains of alkalophilic Bacillus species were obtained and identified as 22 species belonging to 6 genera, Bacillus, Gracilibacillus, Halobacillus, Jeotgalibacillus, Paenibacillus, and Psychrobacillus, by 16S rRNA gene sequences similarities analysis. The genus Bacillus was the dominant bacteria. There were 2 new potential species of alkalophilic Bacillus with 16S rRNA gene similarities 97.00% and 98.65%, respectively. Three enzymes-producing strains accounted for 95.00% of all isolates, including 55 strains with protease activity, 27 strains with cellulase activity, and 8 strains with xylanase. Conclusion] There were rich resources of alkalophilic Bacillus species and abundant enzyme-producing strains in Kekexili of Qinghai Province, which provided a great potential of exploitation of alkaliphilic Bacillus species. |
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| Keywords: | Kekexili Alkaliphilic Bacillus species Protease Cellulase Xylanase |
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