| 瘤胃微生物多样性及功能性研究方法概述 |
| |
| 引用本文： | 李 旦,王加启,卜登攀,刘开朗,赵圣国,于 萍,李长皓,魏宏阳,周凌云.瘤胃微生物多样性及功能性研究方法概述[J].微生物学通报,2008,35(12):1961-1965. |
| |
| 作者姓名： | 李 旦 王加启 卜登攀 刘开朗 赵圣国 于 萍 李长皓 魏宏阳 周凌云 |
| |
| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京,100193
2. 中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京,100193;甘肃农业大学,兰州,730070 |
| |
| 基金项目： | 国家"十一五"科技攻关奶业重大专项，动物营养学国家重点实验室自主研究课题 |
| |
| 摘 要： | 庞大的瘤胃微生物群系之间存在着共生关系,影响着宿主的代谢,是反刍动物营养学的研究热点之一.通过基于16S rRNA的分子生物学方法,如探针法、实时定量PCR法、DGGE/TGGE,RAPD和RFLP技术等研究瘤胃微生物多样性及组成结构,应用宏基因组学如建立YAC文库、BAC文库等研究方法对瘤胃微生物功能特征进行更深入的研究,实现改善反刍动物乳、肉产品品质的目的.
|
| 关 键 词： | 瘤胃微生物 多样性 功能性 研究方法 |
Advances in Methodologies of Ruminant Microbial Ecology and Functionality |
| |
| LI Dan,WANG Jia-Qi,BU Deng-Pan,LIU Kai-Lang,ZHAO Sheng-Guo,YU Ping,LI Chang-Hao,WEI Hong-Yang and ZHOU Ling-Yun.Advances in Methodologies of Ruminant Microbial Ecology and Functionality[J].Microbiology,2008,35(12):1961-1965. |
| |
| Authors: | LI Dan WANG Jia-Qi BU Deng-Pan LIU Kai-Lang ZHAO Sheng-Guo YU Ping LI Chang-Hao WEI Hong-Yang and ZHOU Ling-Yun |
| |
| Institution: | State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193;State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193;State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193;State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193;Gansu Agricultural University, Lanzhou 730070;State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193;State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193;State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193;State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193 |
| |
| Abstract: | There is a symbiotic relationship between the huge ruminant microflora, which plays a crucial role in the metabolic mechanism of ruminant animal. This makes the ruminant microflora as a hotspot. Using methods of molecular biology such as probe methods, Real-time PCR DGGE/TGGE, RAPD and RFLP to study ruminant microbial ecology and metagenomic methods including YAC library and BAC library to study ruminant microflora functionality would attain the aim to approve the quality of milk and meat, meanwhile to explore the abundant resources of ruminant microflora. |
| |
| Keywords: | Ruminant microflora Diversity Functionality Methodology |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《微生物学通报》浏览原始摘要信息 |
| 点击此处可从《微生物学通报》下载免费的PDF全文 |
|
