| 荧光标记法初探植物乳杆菌ST-Ⅲ对Caco-2细胞的粘附机理 |
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| 引用本文： | 陈臣,周方方,吴正钧,王荫榆,陈卫,张灏,郭本恒.荧光标记法初探植物乳杆菌ST-Ⅲ对Caco-2细胞的粘附机理[J].微生物学通报,2010,37(3):0355-0361. |
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| 作者姓名： | 陈臣 周方方 吴正钧 王荫榆 陈卫 张灏 郭本恒 |
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| 作者单位： | 1. 光明乳业股份有限公司,技术中心,乳业生物技术国家重点实验室,上海,200072
2. 江南大学食品学院,食品科学与技术国家重点实验室,江苏,无锡,214122 |
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| 基金项目： | 国家“十一五”科技支撑计划项目(No. 2006BAD04A14) |
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| 摘 要： | 利用荧光探针CFDA-SE标记植物乳杆菌ST-Ⅲ,测定其对Caco-2细胞粘附能力的变化,提取相关物质,探讨其粘附机理。化学和酶处理ST-Ⅲ菌悬液发现,经胃蛋白酶、胰蛋白酶、氯化锂、苯酚、盐酸胍和热处理能显著降低ST-Ⅲ的粘附性,表明表面蛋白或脂磷壁酸(Lipoteichoic acid)可能参与了ST-Ⅲ对Caco-2细胞的粘附。粘附抑制试验和可逆性结合实验证实表面蛋白而非脂磷壁酸参与了ST-Ⅲ对Caco-2细胞的粘附。结果表明ST-Ⅲ的表面蛋白粗提物可能含有粘附素类的S-层蛋白(S-layer protein),经SDS-PAGE电泳分析,此粗提物主要成分的分子量分别为72.7、34.1和24.3kD。
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| 关 键 词： | 植物乳杆菌 粘附 荧光标记 表面蛋白 |
A Fluorescence Labeling Method for the Study on Mechanism of Adhesion of Lactobacillus plantarum ST-Ⅲ on Caco-2 Cells |
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| CHEN Chen,ZHOU Fang-Fang,WU Zheng-Jun,WANG Yin-Yu,CHEN Wei,ZHANG Hao and GUO Ben-Heng.A Fluorescence Labeling Method for the Study on Mechanism of Adhesion of Lactobacillus plantarum ST-Ⅲ on Caco-2 Cells[J].Microbiology,2010,37(3):0355-0361. |
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| Authors: | CHEN Chen ZHOU Fang-Fang WU Zheng-Jun WANG Yin-Yu CHEN Wei ZHANG Hao and GUO Ben-Heng |
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| Institution: | 1. State Key Laboratory of Dairy Biotechnology, Technology Center Bright Dairy & Food Co., Ltd, Shanghai 200072, China;1. State Key Laboratory of Dairy Biotechnology, Technology Center Bright Dairy & Food Co., Ltd, Shanghai 200072, China;1. State Key Laboratory of Dairy Biotechnology, Technology Center Bright Dairy & Food Co., Ltd, Shanghai 200072, China;1. State Key Laboratory of Dairy Biotechnology, Technology Center Bright Dairy & Food Co., Ltd, Shanghai 200072, China;2. State Key Laboratory of Food Science and Technology School of Food Science and Technology Jiangnan University, Wuxi, Jiangsu 214122, China;2. State Key Laboratory of Food Science and Technology School of Food Science and Technology Jiangnan University, Wuxi, Jiangsu 214122, China;1. State Key Laboratory of Dairy Biotechnology, Technology Center Bright Dairy & Food Co., Ltd, Shanghai 200072, China |
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| Abstract: | |
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| Keywords: | Lactobacillus plantarum Adhesion Fluorescence labeling S-layer protein |
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