| 华北地区牛源无乳链球菌的分离鉴定及生物学特性 |
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| 引用本文： | 杜琳,周雪,赵红梅,吕天星,崔健,李松建,史晓娜,郝永清.华北地区牛源无乳链球菌的分离鉴定及生物学特性[J].微生物学通报,2016,43(3):567-574. |
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| 作者姓名： | 杜琳 周雪 赵红梅 吕天星 崔健 李松建 史晓娜 郝永清 |
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| 作者单位： | 内蒙古农业大学兽医学院 内蒙古 呼和浩特 010010,内蒙古农业大学兽医学院 内蒙古 呼和浩特 010010,内蒙古农业大学兽医学院 内蒙古 呼和浩特 010010,内蒙古农业大学兽医学院 内蒙古 呼和浩特 010010,内蒙古农业大学兽医学院 内蒙古 呼和浩特 010010,内蒙古农业大学兽医学院 内蒙古 呼和浩特 010010,内蒙古农业大学兽医学院 内蒙古 呼和浩特 010010,内蒙古农业大学兽医学院 内蒙古 呼和浩特 010010 |
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| 基金项目： | 国家科技支撑计划项目(No. 2012BAD12B09-02) |
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| 摘 要： | 【目的】了解华北地区牛源无乳链球菌的生物学特性。【方法】在2012到2015年间从内蒙古自治区、河北、北京等地隐性乳房炎557份奶牛乳样中分离、收集无乳链球菌。采用纸片扩散法和PCR的方法对这些菌株分别进行耐药谱测定、荚膜分子分型、表面蛋白基因及毒力因子的检测。【结果】无乳链球菌的分离率为5.03%,其药物敏感性与其他地区无明显差别。分离到的28株无乳链球菌均属于荚膜Ia型,且毒力基因基本相同并且其表面蛋白均属于未定型。【结论】华北不同地区的无乳链球菌有相似的药物敏感性和毒力基因。为奶牛乳房炎无乳链球菌疫苗的研制及药物防治提供理论依据。
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| 关 键 词： | 奶牛乳房炎，无乳链球菌，血清型，表面蛋白基因，毒力因子 |
Isolation, identification and characterization of Streptococcus agalactiae of bovine origin in North China |
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| Institution: | Inner Mongolia Agricultural University, College of Veterinary Medicine, Hohhot, Inner Mongolia 010010, China,Inner Mongolia Agricultural University, College of Veterinary Medicine, Hohhot, Inner Mongolia 010010, China,Inner Mongolia Agricultural University, College of Veterinary Medicine, Hohhot, Inner Mongolia 010010, China,Inner Mongolia Agricultural University, College of Veterinary Medicine, Hohhot, Inner Mongolia 010010, China,Inner Mongolia Agricultural University, College of Veterinary Medicine, Hohhot, Inner Mongolia 010010, China,Inner Mongolia Agricultural University, College of Veterinary Medicine, Hohhot, Inner Mongolia 010010, China,Inner Mongolia Agricultural University, College of Veterinary Medicine, Hohhot, Inner Mongolia 010010, China and Inner Mongolia Agricultural University, College of Veterinary Medicine, Hohhot, Inner Mongolia 010010, China |
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| Abstract: | Objective] In order to characterize Streptococcus agalactiae isolates of bovine origin from North China. Methods] Five hundred and fifty-seven milk samples were collected in 2012?2015 from dairy cows with subclinical mastitis in Inner Mongolia Autonomous Region, Hebei Province and Beijing, and S. agalactiae isolates were identified by biochemical analysis and molecular biological methods. Their drug sensitivities were detected using standard disk difusion method, and the genes for capsular type, surface protein and virulence factors were amplified by PCR. Results] Twenty-eight streptococcal isolates were ientified as S. agalactiae with a isolation rate of 5.03% and similar drug sensitivities. All of 28 S. agalactiae isolates belonged to capsular type Ia with similar virulence gene patterns and undefined surface protein types. Conclusion] These data indicate that the S. agalactiae isolates of bovine origin in different regions of North China had similar drug sensitivities and virulence gene patterns, which provided the rationale for development of therapeutic strategy and vaccines against bovine mastitis S. agalactiae. |
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| Keywords: | Dairy cow mastitis Streptococcus agalactiae Serotype Surface protein gene Virulence factor |
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