| 转化异丁香酚生成香草醛纺锤芽孢杆菌的筛选 |
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| 引用本文： | 赵丽青,朱蕾蕾,孙志浩,郑璞.转化异丁香酚生成香草醛纺锤芽孢杆菌的筛选[J].微生物学通报,2006,33(1):72-77. |
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| 作者姓名： | 赵丽青 朱蕾蕾 孙志浩 郑璞 |
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| 作者单位： | 江南大学生物催化研究室,工业生物技术教育部重点实验室,无锡,214036 |
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| 基金项目： | 国家973项目资助（No.2003CB716008） |
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| 摘 要： | 以底物异丁香酚为唯一碳源从七壤中筛选获得了一株能高效转化异丁香酚生成香草醛的芽孢杆菌。根据生理生化特性及16SrRNA序列分析鉴定其属于纺锤芽孢杆菌（Bacillus fusiformis），初步试验表明该菌能转化2％异丁香酚生成4．20g/L香草醚。
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| 关 键 词： | 纺锤芽孢杆菌 异丁香酚 香草醛 分离筛选 鉴定 |
| 文章编号： | 0253-2654（2006）01-0072-06 |
| 收稿时间： | 2005-04-14 |
| 修稿时间： | 2005-06-08 |
Screening and Identification of Bacillus fusiformis Bioconverting Isoeugenol to Vanillin |
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| ZHAO Li-Qing,ZHU Lei-Lei,SUN Zhi-Hao,ZHENG Pu.Screening and Identification of Bacillus fusiformis Bioconverting Isoeugenol to Vanillin[J].Microbiology,2006,33(1):72-77. |
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| Authors: | ZHAO Li-Qing ZHU Lei-Lei SUN Zhi-Hao ZHENG Pu |
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| Institution: | Laboratory of Biocatalysis , School of Bioteehnology , Southern Yangtze University; The Key Laboratory of Industrial Biotechnology, Ministry of Education, Southern Yangtze University, Wuxi 214036 |
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| Abstract: | Using isoeugenol as the sole carbon source, a novel strain, producing high amounts of vanillin from i soeugenol, was isolated from soil. According to the physiological and biochemical characteristics and its 16S rRNA gene sequence analysis, it was identified as Bacillus fusiformis. The initial resulls shewed that 4.20 g/L vanillin was obtained hy bioconversion of 2% isoeugenol with Bacillus fusiformis. |
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| Keywords: | Bacillus fusiformis Isoeugenol Vanillin Screening Identification |
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