|
|||||
|
|
| 产中性纤维素酶特异腐质霉H31-3复合诱变研究 | |
| 引用本文： | 芦敬华,石家骥,葛克山,崔福绵,钱世钧.产中性纤维素酶特异腐质霉H31-3复合诱变研究[J].微生物学通报,2006,33(6):74-78. |
| 作者姓名： | 芦敬华 石家骥 葛克山 崔福绵 钱世钧 |
| 作者单位： | 1. 中国科学院微生物研究所,北京,100080;中国农业大学食品科学与营养工程学院,北京,100083 2. 中国科学院微生物研究所,北京,100080 3. 中国农业大学食品科学与营养工程学院,北京,100083 |
| 基金项目： | 国家十五科技攻关项目（No.2004BA713B03-01）中国科学院知识创新工程重要方向项目（No.KSCX2-SW-324） |
| 摘 要： | 中性纤维素酶在纺织、食品、饲料和制药行业均具有广泛的应用。采用离子束注入技术对中性纤维素酶产生菌特异腐质霉（Humicola insolens）H31-3进行诱变，经发酵筛选获得较高酶活力且传代稳定的正突变菌株H14，制备其原生质体后进行紫外诱变。筛选后得到正突变菌株H14．2，最终CMC酶、滤纸酶活力分别达82．56IU／mL和5．77IU／mL，较原始菌株提高了78．57％和106．81％。 |
| 关 键 词： | 中性纤维素酶 特异腐质霉 离子注入 紫外诱变 |
| 文章编号： | 0253-2654（2006）06-0074-05 |
| 收稿时间： | 2006-02-22 |
| 修稿时间： | 2006-04-12 |
Complex Mutagenesis to Humicola insolens H31-3 Producing Neutral Cellulase |
|
| LU Jing-Hua,SHI Jia-Ji,GE Ke-Shan,CUI Fu-Mian,QIAN Shi-Jun.Complex Mutagenesis to Humicola insolens H31-3 Producing Neutral Cellulase[J].Microbiology,2006,33(6):74-78. | |
| Authors: | LU Jing-Hua SHI Jia-Ji GE Ke-Shan CUI Fu-Mian QIAN Shi-Jun |
| Institution: | Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080;Food Science and Nutrition Engineering Collage, Chinese Agricultural University, Beifing 100083 |
| Abstract: | Neutral cellulase has been broadly used in a lot of industries like food, textile, forage and pharmacy. We screened a positive mutant strain H14 from Humicola insolens H31-3 by ion implantation. And then UV irradiation on the protoplasts from Humicola insolens H14 was performed. After regeneration and screening we obtained another mutant strain, Humicola insolens H14-2, in which CMC enzyme activity and filter paper enzyme activity are raised 78.57% and 106. 81% compared with the parent strain of Humicola insolens H31-3. |
| Keywords: | Neutral cellulase Humicola insolens Ion implantation UV Induced mutation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《微生物学通报》浏览原始摘要信息 | |
| 点击此处可从《微生物学通报》下载免费的PDF全文 | |