| NaCl刺激对乳杆菌冻干存活率及胞内磷酸果糖激酶的影响 |
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| 引用本文： | 张中青,李春,刘宁.NaCl刺激对乳杆菌冻干存活率及胞内磷酸果糖激酶的影响[J].微生物学通报,2011,38(10):1554-1560. |
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| 作者姓名： | 张中青 李春 刘宁 |
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| 作者单位： | 1. 东北农业大学乳品科学教育部重点实验室食品学院,黑龙江哈尔滨,150030
2. 东北农业大学乳品科学教育部重点实验室食品学院,黑龙江哈尔滨,150030;国家乳业工程技术研究中心,黑龙江哈尔滨150086 |
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| 基金项目： | 国家教育部长江学者和创新团队发展计划项目(No. IRT0959); 东北农业大学博士启动基金项目(No. 2010RCB59) |
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| 摘 要： | NaCl刺激影响保加利亚乳杆菌磷酸果糖激酶(PFK)活性及其冻干存活率。在对数期末期保加利亚乳杆菌液中添加2%(W/V)NaCl刺激2 h后,收获菌体,其冻干存活率明显提高。同时采用反相高效液相色谱法(RP-HPLC)对NaCl刺激后的保加利亚乳杆菌胞内PFK活性变化进行测定,NaCl刺激使PFK活性显著增加。利用半定量RT-PCR法对NaCl刺激后PFK基因mRNA表达水平进行了比较和分析,NaCl刺激后冻干前PFK基因的表达量增加,冻干后表达量基本无变化。NaCl刺激能够提高保加利亚乳杆菌的冻干存活率,PFK可能影响保加利亚乳杆菌的存活。
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| 关 键 词： | 保加利亚乳杆菌 冷冻干燥 NaCl刺激 磷酸果糖激酶 半定量RT-PCR |
Effect of NaCl stimulation on the survival rate of freeze-dried cells and intracellular phosphofructokinase in Lactobacillus |
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| ZHANG Zhong-Qing,LI Chun and LIU Ning.Effect of NaCl stimulation on the survival rate of freeze-dried cells and intracellular phosphofructokinase in Lactobacillus[J].Microbiology,2011,38(10):1554-1560. |
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| Authors: | ZHANG Zhong-Qing LI Chun and LIU Ning |
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| Institution: | 1. Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China;1. Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China;1. Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China; 2. National Dairy Engineering & Technical Research Center, Harbin, Heilongjiang 150086, China |
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| Abstract: | NaCl stimulation affects the activity of phosphofructokinase (PFK) and survival rate of freeze-dried Lactobacillus bulgaricus. In the late logarithmic phase, adding 2% (W/V) NaCl to the growth medium markedly increased the survival rate of freeze-dried L. bulgaricus. Meanwhile, the activity of PFK in L. bulgaricus after NaCl stimulation was determined by reversed phase high performance liquid chromatography (RP-HPLC). The results showed that PFK activity was increased significantly by NaCl stimulation. In addition, a semi-quantitative RT-PCR method was applied to detect the PFK gene expression in different condition. The results indicated that the PFK gene expression was increased before freeze-drying and almost unchanged after freeze-drying. NaCl stimulation could enhance the survival rate of freeze-dried cells, and PFK might affect the survival of Lactobacillus bulgaricus. |
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| Keywords: | Lactobacillus bulgaricus Freeze-drying NaCl stimulation Phosphofructokinase Semi-quantitative RT-PCR |
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