| 工业废水中降酚菌的分离及ARDRA多态性分析 |
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| 引用本文： | 陈敏,方序.工业废水中降酚菌的分离及ARDRA多态性分析[J].微生物学通报,2005,32(6):12-15. |
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| 作者姓名： | 陈敏 方序 |
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| 作者单位： | 1. 杭州师范学院生命科学学院,杭州,310012
2. 浙江省微生物研究所,杭州,310012 |
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| 基金项目： | 杭州师范学院重点科研项目（No,2002XNZ02） |
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| 摘 要： | 分别将炼油废水、印染废水、造纸废水样品倍比稀释后涂布平板分离菌株，用苯酚羟化酶基因特异引物检测苯酚降解菌，共分离得到87株降酚菌。经ERIC-PCR指纹图分析，显示15种不同的类型。进一步对显示不同ERIC—PCR指纹图的15种分离物的代表菌株进行ARDRA多态性分析，结果可分为4个OTUs（Operational Taxonomic Unit，OTU），表明实验分离得到的降酚菌至少存在4个不同的种（species）。
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| 关 键 词： | 苯酚降解细菌 苯酚羟化酶基因 ERIC-PCR指纹图 ARDRA多态性分析 |
| 文章编号： | 0253-2654(2005)06-0012-04 |
| 收稿时间： | 01 14 2005 12:00AM |
| 修稿时间： | 02 25 2005 12:00AM |
Isolation of Phenol-degrading Bacteria and Analysis of ARDRA |
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| CHEN Min,FANG Xu.Isolation of Phenol-degrading Bacteria and Analysis of ARDRA[J].Microbiology,2005,32(6):12-15. |
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| Authors: | CHEN Min FANG Xu |
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| Institution: | 1 School of Life Science, Hangzhou Nomal College, Hangzhou 310012 ;2 Zhejiang Institute of Microbiology, Hangzhou 310012 |
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| Abstract: | A number of strains were isolated from the wastewater by direct plating.PCR with specific primer of phenol hydroxylase gene was used to identify the strains as phenol degrading bacteria.By this,a total of 87 phenol degrading strains were purified and divided into populations based on enterobacterial repetitive intergenic consensus sequence PCR(ERIC-PCR) genomic fingerprints.The results indicated that there were 15 different ERIC-PCR types present among the isolates.ARDRA with enzymes AluI revealed 4 operational taxonomic units(OTU) among 15 strains that exhibited different ERIC-PCR types.Moreover,the results indicate that there were 4 species at least. |
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| Keywords: | Phenol hydroxylase gene Phenol-degrading bacterial ERIC-PCR ARDRA |
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