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腾冲热海一株嗜酸热硫化叶菌的分离与鉴定
引用本文:陈 波,魏云林,井申荣,季秀玲,陆月情,林连兵.腾冲热海一株嗜酸热硫化叶菌的分离与鉴定[J].微生物学通报,2008,35(12):1868-1872.
作者姓名:陈 波  魏云林  井申荣  季秀玲  陆月情  林连兵
作者单位:昆明理工大学生物工程技术研究中心,昆明,650224
基金项目:国家自然科学基金资助,云南省自然科学基金资助,中国科学院微生物研究所微生物资源前期开发国家重点实验室开放课题的资助
摘    要:从云南腾冲热海酸性温泉中分离纯化出一株极端嗜酸热菌株K4-1,并对其进行形态观察、生长特征、碳源和能源利用及16S rRNA基因分析.该菌株细胞形态为不规则球形,有单生鞭毛,严格好氧,兼性自养,能利用元素硫作为能源,也能利用酵母膏、精氨酸或核糖作为碳源和能源.其最适生长温度为75℃,最适pH为3.5.通过16S rRNA基因序列相似性对比对该菌株进行鉴定,结果表明该菌株与硫化叶茵属标准菌株的相似性介于86.6%~94.3%之间,与分离自腾冲热海的腾冲硫化叶菌Sulfolobus tengchongensis RT8-4菌株序列相似性最高,达到98.9%,可将菌株K4-1鉴定为硫化叶菌属菌株.菌株K4-1的16S rRNA基因序列号为EU729124.

关 键 词:极端嗜热古菌  硫化叶菌  系统进化分析  分离鉴定

Identification of a Thermoacidophilic Sulfolobus sp. Isolated from a Hot Spring in Tengchong Rehai
CHEN Bo,WEI Yun-Lin,JING Shen-Rong,JI Xiu-Ling,LU Yue-Qing and LIN Lian-Bing.Identification of a Thermoacidophilic Sulfolobus sp. Isolated from a Hot Spring in Tengchong Rehai[J].Microbiology,2008,35(12):1868-1872.
Authors:CHEN Bo  WEI Yun-Lin  JING Shen-Rong  JI Xiu-Ling  LU Yue-Qing and LIN Lian-Bing
Institution:Biotechnology Research Center, Kunming University of Science and Technology, Kunming 560224;Biotechnology Research Center, Kunming University of Science and Technology, Kunming 560224;Biotechnology Research Center, Kunming University of Science and Technology, Kunming 560224;Biotechnology Research Center, Kunming University of Science and Technology, Kunming 560224;Biotechnology Research Center, Kunming University of Science and Technology, Kunming 560224;Biotechnology Research Center, Kunming University of Science and Technology, Kunming 560224
Abstract:An extremely thermoacidophilic isolate K4-1 was obtained from an acidic hot spring in Tengchong Rehai, Yunnan province. Morphology, growth characteristics, utilization of carbon compounds, energy sources and 16S rRNA gene sequence of K4-1 were studied. Cells of K4-1 are irregular cocci with monotrichous flagella. The strain grew aerobically in either a lithotrophic or a heterotrophic mode. Growth on elemental sulfur occurred through oxidation of sulfur. It grew optimally at 75°C and pH 3.5. On the basis of 16S rRNA gene sequence similarity, strain K4-1 was shown to belong to genus Sulfolobus, being related to the type strains of genus Sulfolobus (86.6%~94.3% similarity), and being most closely related to strain Sulfolobus tengchongensis RT8-4 (98.9% similarity). The GenBank accession number of strain K4-1 16S rRNA gene sequence is EU729124.
Keywords:Thermoacidophilic archaeon  Sulfolobus  Phylogenetic analysis  Identification
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