| 玉米大斑病菌Ht—毒素的萃取及其致病活性 |
| |
| 引用本文： | 董金皋, 史有艳, 康绍兰, 黄梧芳,.玉米大斑病菌Ht—毒素的萃取及其致病活性[J].微生物学通报,1993,20(2):73-77. |
| |
| 作者姓名： | 董金皋 史有艳 康绍兰 黄梧芳 |
| |
| 作者单位： | 河北农业大学植物保护系 保定; |
| |
| 摘 要： | 采用体外(in vitro)固体和液体培养玉米大斑病菌——大斑病长晨蠕孢(Helminthosporium turcicum),培养物经有机溶剂萃取后减压蒸发获得了Ht-粗毒素,最后用种子根伸长抑制,离体根冠细胞死亡和离体叶片致萎等生物测定法,对玉米大斑病菌的体外代谢产物进行了致病活性和毒性测定,筛选出乙腈等有机溶剂,可以用于Ht-毒素的萃取,为Ht-毒素的进一步提纯和结构分析奠定了基础。
|
| 关 键 词： | 玉米 大斑病长蠕孢（Helminthosporium turcicum） 真菌毒素 毒素萃取 生物测定 |
EXTRACTION OF HT-TOXIN PRODUCED BY HELMINTHOSPORIUM TURCICUM AND ITS PATHOGENIC ACTIVITY |
| |
| Dong Jingao Shi Youyan,Kang Shaolan Huang Wufang.EXTRACTION OF HT-TOXIN PRODUCED BY HELMINTHOSPORIUM TURCICUM AND ITS PATHOGENIC ACTIVITY[J].Microbiology,1993,20(2):73-77. |
| |
| Authors: | Dong Jingao Shi Youyan Kang Shaolan Huang Wufang |
| |
| Abstract: | Ht-toxin was extracted by singular or mixed organic solvents after solid and liquid culturing Helminthosporium turcicum in vitro, and pathogenic activity and toxicity of these extracts were assayed using root growth inhibition, isolated root cap cell death and isolated leaf soaking bioassays. Several best singular and mixed organic solvents have been screened for extracting Ht-toxin in vitro and this may lay foundation for its purification and structure analysis. |
| |
| Keywords: | Helminthosporium turcicum Mycotoxin Bioassay Extraction of Ht-toxin Maize |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《微生物学通报》浏览原始摘要信息 |
| 点击此处可从《微生物学通报》下载免费的PDF全文 |
|
