| 腾冲两热泉泉古菌多样性及系统发育的初步分析 |
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| 引用本文： | 宋兆齐,陈经全,职晓阳,黄志勇,张传伦,李文均.腾冲两热泉泉古菌多样性及系统发育的初步分析[J].微生物学通报,2008,35(3):0372-0377. |
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| 作者姓名： | 宋兆齐 陈经全 职晓阳 黄志勇 张传伦 李文均 |
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| 作者单位： | 1. 云南大学,云南省微生物研究所,教育部微生物多样性可持续利用重点实验室,昆明,650091
2. Department of Marine Sciences,University of Georgia,Athens,GA 30602,USA |
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| 基金项目： | 国家重点基础研究发展计划(973计划)
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国家自然科学基金
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教育部跨世纪优秀人才培养计划
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云南省中青年学术技术带头人后备人才专项基金 |
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| 摘 要： | 通过构建16S rRNA基因片段的克隆文库对腾冲热海两温泉中泉古菌的多样性和系统发育关系进行了初步的研究.一共得到18个泉古茵克隆序列,可分为12个OTUs,两温泉的克隆序列与已知GenBank上关系最近序列的平均相似性较低,无名泉为92.56%,热爆区为93%.从基于16S rRNA基因片段序列构建的系统发育树来看,74℃的无名泉样点中既有属于超高温环境类群的泉古菌,同时又有属于和常温环境较接近的泉古菌;45℃的热爆区样点的泉古茵,相对来说则更接近于常温类群.本次研究表明,腾冲热泉与世界其它同类热泉之间的泉古茵类群存在着一定的差异;而且两实验样点代表了超高温和高温环境泉古菌逐渐向常温过度的两个重要环境.
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| 关 键 词： | 泉古菌 多样性 系统发育 16S rRNA基因 克隆文库 热泉 腾冲 腾冲热海 热泉 古菌 系统发育树 分析 Tengchong Hot Springs Phylogenetic Analysis 常温环境 超高温 代表 实验 差异 存在 世界 类群 高温环境 基因片段序列 热爆 名泉 |
| 修稿时间： | 2007年7月30日 |
Crenarchaeal Diversity and Phylogenetic Analysis of Two Hot Springs in Tengchong |
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| SONG Zhao-Qi,CHEN Jing-Quan,ZHI Xiao-Yang,HUANG Zhi-Yong,ZHANG Chuan-Lun and LI Wen-Jun.Crenarchaeal Diversity and Phylogenetic Analysis of Two Hot Springs in Tengchong[J].Microbiology,2008,35(3):0372-0377. |
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| Authors: | SONG Zhao-Qi CHEN Jing-Quan ZHI Xiao-Yang HUANG Zhi-Yong ZHANG Chuan-Lun and LI Wen-Jun |
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| Institution: | The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091;The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091;The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091;Department of Marine Sciences, University of Georgia, Athens, GA 30602, USA;Department of Marine Sciences, University of Georgia, Athens, GA 30602, USA;The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091 |
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| Abstract: | The crenarchaeal diversity and phylogenesis of two hot springs in Tengchong were investigated and analyzed using the construction of 16S rRNA gene clone libraries. Total 18 crenarchaeal sequences were obtained and divided into 12 OTUs. The average similarities between the clone sequences from Wuming hot spring and Rebao hot spring and their closest sequences deposited in GenBank is 92.56% and 93%, respectively. Based on the phylogenetic tree constructed by using crenarchaeal 16S rRNA gene sequences, Wuming hot spring not only have hyperthethermophilic crenarchaeal clusters but also have the crenarchaeal clusters that have close phylogenetic relationship with non-thermophilic crenarchaeota. And most of the crenarchaeota in Rebao spring distribute in normal temperature groups. This study showed that crenarchaeal clusters in Tengchong hot springs have some differences with other hot springs in the world, and these springs represent two important environments for investigating the evolutionary origin of the non-thermophilic Crenarchaeoone library. |
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| Keywords: | Crenarchaeota Diversity Phylogenesis 16S rRNA gene Clone library Hot spring Tengchong |
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