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| 棉花根际亲和性高效促生细菌的分离筛选 | |
| 引用本文： | 夏觅真,马忠友,齐飞飞,常慧萍,唐欣昀,甘旭华.棉花根际亲和性高效促生细菌的分离筛选[J].微生物学通报,2008,35(11):1738-1743. |
| 作者姓名： | 夏觅真 马忠友 齐飞飞 常慧萍 唐欣昀 甘旭华 |
| 作者单位： | 1. 安徽农业大学生命科学学院,合肥,230036;安徽医科大学,合肥,230032 2. 安徽农业大学生命科学学院,合肥,230036;安徽科技学院,凤阳,233100 3. 安徽农业大学生命科学学院,合肥,230036 |
| 基金项目： | 安徽省科技创新项目，安徽省科技厅年度重点项目 |
| 摘 要： | 为了从棉花根际土壤筛选能与棉花凝集素具有亲和作用的高效促生细菌,以选择性培养基从棉花根部初步筛选具有固氮能力、解磷能力及解钾能力的促生细菌,再以异硫氰酸磺(FITC)标记的棉花凝集素为复筛工具,从棉花根际促生细菌中筛选能与棉花凝集素结合的亲和性菌株,分别挑选2株固氮菌、2株解磷细菌和2株解钾细菌作为微生物肥料接种到棉花根部进行盆栽试验.观察其在根部定殖情况.结果是在选择性平板上有20%～30%的菌株具有凝集素染色阳性.盆栽试验显示,接种的6株亲和性菌株能在棉花根部成功定殖,根际细菌数量约是灭活对照的`0倍.通过初步鉴定,固氮菌株N1111为固氮菌属(Azotobacter),N2121属于德克斯氏菌属(Derxia);解磷菌株P2126属于黄单胞菌属(Xanthomonas),P1108菌株为假单胞菌属(Pseudomonas);解钾菌株K2204和K2116属于芽孢杆菌属(Bacillus). |
| 关 键 词： | 棉花凝集素 促生细菌 亲和性菌株 盆栽试验 |
Isolation and Screening of Effective and Affinity Strains of Growth-Promoting Bacteria from Cotton Rhizosphere |
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| XIA Mi-Zhen,MA Zhong-You,QI Fei-Fei,CHANG Hui-Ping,TANG Xin-Yun and GAN Xu-Hua.Isolation and Screening of Effective and Affinity Strains of Growth-Promoting Bacteria from Cotton Rhizosphere[J].Microbiology,2008,35(11):1738-1743. | |
| Authors: | XIA Mi-Zhen MA Zhong-You QI Fei-Fei CHANG Hui-Ping TANG Xin-Yun and GAN Xu-Hua |
| Institution: | (1. Anhui Agricultural University, Hefei 230036)(2. Anhui Medical University, Hefei 230032);(1. Anhui Agricultural University, Hefei 230036) (3. Anhui Science and Technology College, Fengyang 233100);Anhui Agricultural University, Hefei 230036;Anhui Agricultural University, Hefei 230036;Anhui Agricultural University, Hefei 230036;Anhui Agricultural University, Hefei 230036 |
| Abstract: | To screen effective and affinity plant growth-promoting rhizobacteria strains(PGPR) that can react with cotton lectin from cotton rhizosphere,we isolate nitrogen-fixation bacteria,phosphate-releasing bacteria and potassium-releasing bacteria from cotton rhizosphere by Ashby medium,phosphate-releasing bacteria medium and potassium-releasing bacteria medium,respectively.The strains were re-screened by cotton lectin labeled with fluorescein isothiocyanate.According to their ability of nitrogen-fixation,p-relea... |
| Keywords: | Cotton lectin Plant growth-promoting rhizobacteria Affinity strain Pot experiment |
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