首页 | 本学科首页   官方微博 | 高级检索  
   检索      

新疆泥火山细菌群落PCR-SSCP 分析
引用本文:路盼盼,李建辉,张亚平.新疆泥火山细菌群落PCR-SSCP 分析[J].微生物学通报,2011,38(2):195-198.
作者姓名:路盼盼  李建辉  张亚平
作者单位:石河子大学生命科学学院,新疆石河子,832003
基金项目:国家自然科学基金项目(No. 30860001)
摘    要:采用单链构象多态性(SSCP)技术, 以16S rDNA 基因的V3 区为靶对象, 分析泥火山细菌多样性及其群落结构。通过对泥火山不同月份的不同深度土样DNA 提取后, 针对16S rDNA 进行PCR 扩增出236 bp 大小片段, 通过SSCP 电泳对泥火山细菌进行季节性多样性分析, 并对主要条带进行克隆分析。结果显示: 新疆泥火山细菌不同季节多态性明显, 而且这种多态性容易受生态和气候的影响; 假单胞菌属是泥火山土壤优势菌群, 在泥火山区土壤中广泛分布, 受生态和气候环境影响较小。

关 键 词:泥火山    PCR-SSCP    16S  rDNA
收稿时间:9/7/2010 12:00:00 AM
修稿时间:2010/11/23 0:00:00

PCR-SSCP analysis bacterial community of Mud Volcano in Xinjiang
LU Pan-Pan,LI Jian-Hui and ZHANG Ya-Ping.PCR-SSCP analysis bacterial community of Mud Volcano in Xinjiang[J].Microbiology,2011,38(2):195-198.
Authors:LU Pan-Pan  LI Jian-Hui and ZHANG Ya-Ping
Institution:College of Life Science, Shihezi University, Shihezi, Xinjiang 832003, China;College of Life Science, Shihezi University, Shihezi, Xinjiang 832003, China;College of Life Science, Shihezi University, Shihezi, Xinjiang 832003, China
Abstract:A technology sigle-strand conformation polymorphism (SSCP) and a target 16S rDNA gene on V3 region were used to analyze mud volcano microbial community and structure. After extracting DNA samples from different mud deepth and month, get a 236 bp fragent by PCR. The fragment was used to analyze the microorganisms seasonal diversity of mud volcano by SSCP, and some target feagments were analyzed clone. Resluts showed: the bacteria polymorphism in Xijiang mud volcano soil is significant, which is vulnerable to ecological and climatics factors. Pseudomonas is a dominant microorganism in Xijiang mud volcano, it distributes widely in mud volcano and was affected little by ecological and climatics.
Keywords:Mud volcano  PCR-SSCP  16S rDNA
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《微生物学通报》浏览原始摘要信息
点击此处可从《微生物学通报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号