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| 一株瘤胃纤维素降解菌的分离鉴定及其纤维素降解特性 | |
| 引用本文： | 刘占英,侯先志,刘玉承,刘小刚,胡建华,杨 帆,赵子夫.一株瘤胃纤维素降解菌的分离鉴定及其纤维素降解特性[J].微生物学通报,2009,36(3):0459-0464. |
| 作者姓名： | 刘占英 侯先志 刘玉承 刘小刚 胡建华 杨 帆 赵子夫 |
| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018;内蒙古工业大学化工学院,内蒙古,呼和浩特,010051 2. 内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018 3. 内蒙古工业大学化工学院,内蒙古,呼和浩特,010051 |
| 基金项目： | 国家自然科学基金资助项目(No. 30460095) |
| 摘 要： | 从蒙古绵羊瘤胃内容物中分离到一株纤维素降解细菌WH-1, 通过形态、生理生化特征、G+C mol%含量和16S rRNA序列分析对分离菌株进行鉴定, 鉴定为溶纤维丁酸弧菌属(Butyrivibrio fibrisolvens)的溶纤维丁酸弧菌(Butyrivibrio fibrisolvens)。同时, 用Mega 4.1软件构建的系统发育树显示分离菌株WH-1与多株溶纤维丁酸弧菌(Butyrivibrio fibrisolvens)的亲缘关系最近。对该菌株纤维素降解特性的初步研究表明：当温度为37°C、 |
| 关 键 词： | 瘤胃纤维素降解细菌 溶纤维丁酸弧菌 |
Isolation, Identification and Characterization of a Cellulose-degrading Bacterial Strain from the Rumen of Sheep |
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| LIU Zhan-Ying,HOU Xian-Zhi,LIU Yu-Cheng,LIU Xiao-Gang,HU Jian-Hu,YANG Fan and ZHAO Zi-Fu.Isolation, Identification and Characterization of a Cellulose-degrading Bacterial Strain from the Rumen of Sheep[J].Microbiology,2009,36(3):0459-0464. | |
| Authors: | LIU Zhan-Ying HOU Xian-Zhi LIU Yu-Cheng LIU Xiao-Gang HU Jian-Hu YANG Fan and ZHAO Zi-Fu |
| Institution: | (1. College of Animal Science and Animal Medicine, Inner Mongolia University of Agriculture, Hohhot, Inner Mongolia 010018, China) (2. College of Chemical Engineering, Inner Mongolia University of Technology, Hohhot, Inner Mongolia 010051, China);College of Animal Science and Animal Medicine, Inner Mongolia University of Agriculture, Hohhot, Inner Mongolia 010018, China;College of Animal Science and Animal Medicine, Inner Mongolia University of Agriculture, Hohhot, Inner Mongolia 010018, China;College of Animal Science and Animal Medicine, Inner Mongolia University of Agriculture, Hohhot, Inner Mongolia 010018, China;College of Chemical Engineering, Inner Mongolia University of Technology, Hohhot, Inner Mongolia 010051, China;College of Animal Science and Animal Medicine, Inner Mongolia University of Agriculture, Hohhot, Inner Mongolia 010018, China;College of Animal Science and Animal Medicine, Inner Mongolia University of Agriculture, Hohhot, Inner Mongolia 010018, China |
| Abstract: | A bacterial strain named as WH-1 that can degrade cellulose was isolated from the rumen of the Mongolia sheep. The isolate was identified as Butyrivibrio fibrisolvens based on phenotypic and morphologic properties, the G+C mol% content of DNA and the 16S rRNA gene sequence analyses. In addition, the phylogenetic analysis of the 16S rRNA sequence also indicated that the isolate grouped closely with other members of Butyrivibrio fibrisolvens. The primary factors affecting the degradative ratio of cellulose were examined. The highest degradative ratio of filter paper reached 16.81%±2.99% under the conditions of 37°C, pH 7.0, inoculum size being 25%, and the percentage of cellobiose in total carbon sources being 20% within 72 hours of fermentation. |
| Keywords: | Cellulose-degrading rumen bacterium Butyrivibrio fibrisolvens |
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