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工业用糖蜜酿酒酵母菌株耐受性分析研究
引用本文:郭 亭,梁达奉.工业用糖蜜酿酒酵母菌株耐受性分析研究[J].微生物学通报,2008,35(2):0188-0192.
作者姓名:郭 亭  梁达奉
作者单位:广州甘蔗糖业研究所,广东省甘蔗改良与生物炼制重点实验室,广州,510316
摘    要:采用休止细胞梯度生长法,对工业糖蜜酿酒酵母(Saccharomyces cerevisiae)菌株进行高浓度酒精、高温和高渗透压,以及糠醛毒性、苯酚毒性、乙酸毒性和抗生素G418毒性的耐受性分析.结果表明,所测定的工业酵母菌株对这些逆境条件的耐受性有明显的差别;其中AS2.1189和AS2.1190对测定的胁迫条件均表现出相对较好的耐受性;396对乙酸毒性和G418毒性具有很好的耐受性;2610对高温表现出较强的耐受性.

关 键 词:酿酒酵母  耐受性  燃料乙醇  工业用  糖蜜  酿酒酵母  工业酵母菌株  耐受性  分析  研究  Strains  Industrial  Saccharomyces  cerevisiae  Stress  Resistance  表现  胁迫条件  差别  测定  结果  抗生素  乙酸  苯酚  毒性
修稿时间:2007年6月20日

Research on the Stress Resistance of Saccharomyces cerevisiae Industrial Strains for Molasses
GUO Ting and LIANG Da-Feng.Research on the Stress Resistance of Saccharomyces cerevisiae Industrial Strains for Molasses[J].Microbiology,2008,35(2):0188-0192.
Authors:GUO Ting and LIANG Da-Feng
Institution:Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou 510316;Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou 510316
Abstract:The stress resistance of Saccharomyces cerevisiae industrial strains for molasses to highconcentration ethanol, high temperature, high osmotic pressure, furfural toxicity, phenol toxicity, acetic acid toxicity and G418 toxicity were analyzed by the spot dilution growth assays in this paper. The results showed that the stress resistances among these industrial strains were obviously different. The strains AS2.1189 and AS2.1190 are more resistant to the tested stress factors than any others .The strain 396 is the most resistant to the acetic acid toxicity and G418 toxicity, and the strain 2610 is the most resistant to the high temperature.
Keywords:Saccharomyces cerevisiae  Stress resistance  Fuel ethanol
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