| 志贺氏菌福氏2a 301株ipaH4.5突变株的构建及生物学功能分析 |
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| 引用本文： | 王芳,赵江丽,何湘,姜铮,刘大伟,陈宣楠,袁静,黄留玉.志贺氏菌福氏2a 301株ipaH4.5突变株的构建及生物学功能分析[J].微生物学通报,2010,37(5):0714-0720. |
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| 作者姓名： | 王芳 赵江丽 何湘 姜铮 刘大伟 陈宣楠 袁静 黄留玉 |
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| 作者单位： | 1. 军事医学科学院疾病预防控制所,北京,100071;解放军第309医院,北京,100091
2. 军事医学科学院疾病预防控制所,北京,100071 |
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| 摘 要： | 利用λ-Red重组系统对福氏2a志贺氏菌301株ipaH4.5基因进行缺失突变,构建了福氏2a志贺氏菌301株ipaH4.5基因缺失突变株?ipaH4.5,利用低拷贝质粒构建ipaH4.5缺失突变株的回复突变株△ipaH4.5HF。PCR方法证实了ipaH4.5基因的缺失和回复。对野生株、突变株和回复突变株的生长代谢及细胞侵袭能力进行比较;ELISA方法检测3株菌侵袭鼠J774巨噬细胞后培养上清中炎性因子的水平。生长代谢实验表明缺失和回复ipaH4.5不影响志贺氏菌的生长速度,侵袭实验表明缺失和回复ipaH4.5也不影响志贺氏菌对HeLa细胞和鼠J774巨噬细胞的侵袭能力,表明ipaH4.5基因与志贺氏菌的生长代谢和侵袭能力无关;鼠J774巨噬细胞培养上清中细胞因子水平的改变提示该基因在志贺氏菌侵入细胞后抑制宿主细胞炎症反应。
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| 关 键 词： | 福氏志贺氏菌 ipaH4.5 基因缺失 生物学功能 侵袭实验 |
Construction and Function Analysis of the ipaH4.5 Gene Deletion Mutant of Shigella flexneri 2a Strain 301 |
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| WANG Fang,ZHAO Jiang-Li,HE Xiang,JIANG Zheng,LIU Da-Wei,CHEN Xuan-Nan,YUAN Jing and HUANG Liu-Yu.Construction and Function Analysis of the ipaH4.5 Gene Deletion Mutant of Shigella flexneri 2a Strain 301[J].Microbiology,2010,37(5):0714-0720. |
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| Authors: | WANG Fang ZHAO Jiang-Li HE Xiang JIANG Zheng LIU Da-Wei CHEN Xuan-Nan YUAN Jing and HUANG Liu-Yu |
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| Institution: | 1. Institute of Disease Prevention and Control, Academy of Military Medical Sciences, Beijing 100071, China; 2. No. 309th Hospital of Chinese PLA, Beijing 100091, China;1. Institute of Disease Prevention and Control, Academy of Military Medical Sciences, Beijing 100071, China;1. Institute of Disease Prevention and Control, Academy of Military Medical Sciences, Beijing 100071, China;1. Institute of Disease Prevention and Control, Academy of Military Medical Sciences, Beijing 100071, China;1. Institute of Disease Prevention and Control, Academy of Military Medical Sciences, Beijing 100071, China;1. Institute of Disease Prevention and Control, Academy of Military Medical Sciences, Beijing 100071, China;1. Institute of Disease Prevention and Control, Academy of Military Medical Sciences, Beijing 100071, China;1. Institute of Disease Prevention and Control, Academy of Military Medical Sciences, Beijing 100071, China |
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| Abstract: | |
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| Keywords: | shigella flexneri 2a IpaH4 5 gene gene deletion Biological function invasion assay |
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