| 烟草炭疽病拮抗生防菌的筛选 |
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| 引用本文： | 万秀清,郭兆奎,颜培强,李洪林,李丽杰,于红梅.烟草炭疽病拮抗生防菌的筛选[J].微生物学通报,2008,35(11):1727-1731. |
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| 作者姓名： | 万秀清 郭兆奎 颜培强 李洪林 李丽杰 于红梅 |
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| 作者单位： | 1. 黑龙江省烟草科学研究所,牡丹江,157011
2. 牡丹江市北山公园,牡丹江,157011 |
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| 基金项目： | 黑龙江省烟草专卖局资助项目"烟草病虫害生物防治技术研究" |
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| 摘 要： | 本文应用荧光假单胞杆菌的16S-23S ribosomal RNA序列以及部分抗菌素合成基因序列的PCR鉴定引物,对从烟草根际分离到的400份细菌进行了鉴定,结果获得了21份含Prn(硝吡咯菌素)合成酶基因的荧光假单胞杆菌和1份含有DAPG(2,4-二乙酰基藤黄酚)合成酶基因以及PCA(Phenazine-1-carboxylic acid,吩嗪-1-羧酸)的荧光假单胞杆菌.室内实验结果表日月,含DAPG和PCA的荧光假单胞杆菌对烟草炭疽病具有明显拮抗作用.
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| 关 键 词： | 荧光假单胞杆菌 烟草炭疽病 生防 |
Screening of Antagonistic Strains to Tobacco Anthracnose |
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| WAN Xiu-Qing,GUO Zhao-Kui,YAN Pei-Qiang,LI Hong-Lin,LI Li-Jie and YU Hong-Mei.Screening of Antagonistic Strains to Tobacco Anthracnose[J].Microbiology,2008,35(11):1727-1731. |
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| Authors: | WAN Xiu-Qing GUO Zhao-Kui YAN Pei-Qiang LI Hong-Lin LI Li-Jie and YU Hong-Mei |
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| Institution: | Heilongjiang Tobacco Research Institute, Mudanjiang 157011;Heilongjiang Tobacco Research Institute, Mudanjiang 157011;Heilongjiang Tobacco Research Institute, Mudanjiang 157011;Heilongjiang Tobacco Research Institute, Mudanjiang 157011;Heilongjiang Tobacco Research Institute, Mudanjiang 157011;Mudanjiang Beishan Park, Mudanjiang 157011 |
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| Abstract: | Special PCR primers were designed from the sequence of 16S-23S ribosomal RNA of Pseudomonas fluorescens, then these primers were used to screening 400 bacteria strains which selected from tobacco planting soil. In the experiment, 21 Pseudomonas fluorescens strains which contain Pyrrolnitrin had been identified, and 1 Pseudomonas fluorescens strain which contain 2,4-diacetylphloroglucinal and Phenazine-1-carboxylic acid had been identified, too. The results showed that the strain which contain 2,4-diacetylphloroglucinal and Phenazine-1-carboxylic acid inhibit to tobacco anthracnose. |
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| Keywords: | Pseudomonas fluorescens Tobacco anthracnose Antagonism |
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