| 驱除痢疾杆菌侵袭大质粒的新方法 |
| |
| 引用本文： | 冯尔玲, 廖翔, 王恒樑, 史兆兴, 苏国富, 黄留玉,.驱除痢疾杆菌侵袭大质粒的新方法[J].微生物学通报,2003,30(3):69-73. |
| |
| 作者姓名： | 冯尔玲 廖翔 王恒樑 史兆兴 苏国富 黄留玉 |
| |
| 作者单位： | 军事医学科学院生物工程研究所,北京,100071 |
| |
| 摘 要： | 用质粒不相容性原理驱除痢疾杆菌福氏 2a 2 4 5 7T和宋内S7的侵袭大质粒 ,先从福氏2a侵袭大质粒分别扩增ori和inc基因 ,将它们克隆至 pMD18 T载体 ,得重组质粒pMDori和 pMDinc ,然后转化 2 4 5 7T和S7,不管是pMDori还是 pMDinc都能竞争驱除痢疾杆菌福氏 2a 2 4 5 7T和宋内S7的侵袭大质粒。
|
| 关 键 词： | 痢疾杆菌 侵袭大质粒 质粒不相容性 |
| 文章编号： | 0253-2654(2003)03-0069-05 |
| 修稿时间： | 2002年7月9日 |
THE REMOVAL OF LARGE INVASIVE PLASMIDS FROM SHIGELLA SPECIES |
| |
| FENG Er,Ling,LIAO Xiang,WANG Heng,Liang,SHI Zhao,Xing,SU Guo,Fu,HUANG Liu,Yu.THE REMOVAL OF LARGE INVASIVE PLASMIDS FROM SHIGELLA SPECIES[J].Microbiology,2003,30(3):69-73. |
| |
| Authors: | FENG Er Ling LIAO Xiang WANG Heng Liang SHI Zhao Xing SU Guo Fu HUANG Liu Yu |
| |
| Abstract: | In order to remove invasive plasmids from Shigella flexneri 2a 2457T and Shigella sonnei S7 based on the principle of plasmid incompatibility Ori and inc genes were amplified by PCR from S. flexneri 2a invasive plasmids, and then they were cloned into plasmid pMD18 T, resulting recombinant plasmids pMDori and pMDinc After S flexneri 2a 2457T and S sonnei S7 were transformed with pMDori or pMDinc respectively Invasive plasmids were removed from S flexneri 2a 2457T and S sonnei S7 |
| |
| Keywords: | Shigella species Invasive plasmid Incompatibility |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《微生物学通报》浏览原始摘要信息 |
| 点击此处可从《微生物学通报》下载免费的PDF全文 |
|
