| 特定双歧杆菌菌株对炎症性肠病小鼠的影响 |
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| 引用本文： | 金丹,孟繁平,崔逢德,李红花,金寿起.特定双歧杆菌菌株对炎症性肠病小鼠的影响[J].微生物学通报,2011,38(3):383-387. |
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| 作者姓名： | 金丹 孟繁平 崔逢德 李红花 金寿起 |
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| 作者单位： | 1. 延边大学基础医学院免疫学与病原生物学教研部,吉林延吉,133000
2. 韩国延世大学原州医科大学微生物学教研室,韩国,原州 |
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| 摘 要： | 为了观察特定双歧杆菌菌株对葡聚糖硫酸钠(DSS)诱导的Balb/c小鼠结肠炎的影响,探讨该双歧杆菌菌株对炎症性肠病(IBD)的防治作用及其可能机制。将小鼠分为3组:正常对照组、模型组(DSS组)、实验组(DSS+Bf组)。用已挑选好的双歧杆菌菌株预先处理小鼠4周后,用4%DSS溶液诱导急性结肠炎。实验结束后,检测每组小鼠结肠的长度及结肠炎炎症程度,利用半定量RT-PCR法检测小鼠肠道派伊尔结细胞中IL-10的表达,利用免疫组化实验检测肠道黏膜中IL-10分泌阳性细胞。结果实验组小鼠结肠的平均长度为7.80 cm±0.21 cm,虽较正常对照组(9.10 cm±0.82 cm)缩短,但较模型组(6.80 cm±0.31 cm)其缩短程度减少;结肠炎炎症程度肉眼评分结果:模型组和实验组分别为8.60±0.24分和6.60±0.68分,两组之间均有显著性差异(P<0.05),显微镜下评分结果为实验组(6.80±0.73分)较模型组(8.80±0.37分)明显减少(P<0.05);实验组小鼠肠道派伊尔结细胞IL-10的表达和肠道黏膜IL-10分泌阳性细胞数明显多于模型组。该实验所用的双歧杆菌菌株减轻了DSS诱导的小鼠肠道炎症反应,并且增强了肠道免疫细胞的IL-10的表达及分泌。
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| 关 键 词： | 双歧杆菌 炎症性肠病 白介素-10 |
| 收稿时间： | 2010/7/22 0:00:00 |
| 修稿时间： | 2010/11/25 0:00:00 |
Effect of specific Bifidobacteria strain on the inflammatory bowel disease mice |
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| JIN Dan,MENG Fan-Ping CUI Feng-De LI Hong-Hua JIN Shou-Qi,CUI Feng-De LI Hong-Hua JIN Shou-Qi,LI Hong-Hua JIN Shou-Qi and JIN Shou-Qi.Effect of specific Bifidobacteria strain on the inflammatory bowel disease mice[J].Microbiology,2011,38(3):383-387. |
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| Authors: | JIN Dan MENG Fan-Ping CUI Feng-De LI Hong-Hua JIN Shou-Qi CUI Feng-De LI Hong-Hua JIN Shou-Qi LI Hong-Hua JIN Shou-Qi and JIN Shou-Qi |
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| Institution: | Department of Immunology and Pathogenic Biology, Yanbian University College of Basic Medicine, Yanji, Jilin 133000, China;Department of Immunology and Pathogenic Biology, Yanbian University College of Basic Medicine, Yanji, Jilin 133000, China;Department of Immunology and Pathogenic Biology, Yanbian University College of Basic Medicine, Yanji, Jilin 133000, China;Department of Immunology and Pathogenic Biology, Yanbian University College of Basic Medicine, Yanji, Jilin 133000, China;Department of Department of Microbiology, Wonju College of Medicine, Yonsei University, Wonju, Korea |
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| Abstract: | In the present paper we investigated the effects of specific bifidobacteria strain as supplemen-tation on acute inflammatory bowel disease(IBD) induced by dextran sulfate sodium(DSS) and the pos-sible mechanism was also discussed.Mice were divided into three groups as control,DSS,and experiment(DSS+Bf) group.The mice in DSS group were fed with 4% DSS(W/V) solution for 7 days to induce coli-tis,and the mice in DSS+Bf group,were then given bifidobacteria by oral gavage for next 4 weeks.Both the colonic length... |
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| Keywords: | Bifidobacteria IBD IL-10 |
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