结核分枝杆菌临床株4种耐药基因的检测与分析

结核分枝杆菌Mycobacterium tuberculsis(M.t)4种耐药基因的研究，了解耐药基因突变情况和耐药水平的关系。108例临床痰标本临床分离株均做传统梯度药敏试验和聚合酶链反应多态-单链构象多态性（PCR-SSCP）试验。结果表明耐SM（rpsL）REP(rpoB）INH(katG）EMB（embB）基因突变率分别为78.5%,68.2%，70.5%，48.6%。其中，上海高耐药株基因突变率分别为86.5%，89.3%，84.3%，35.3%。低耐药株分别为28.5%，16.5%，7.1%。EMB在低耐药区无基因突变。M.t的4种耐药基因联合检测的分析，在国内外很少报道。部分M.t的耐药由耐药基因突变所致，M.t耐药基因突变与耐药水平密切相关，且M.t基因突变绝大多数发生在高耐药区中，也有少部分在低耐区株中发生。

THE RESEARCH OF FOUR DRUG RESISYANCE GENE IN MYCOBACTERIUM TUBERCULOSIS

objective To study the reationship beween drug-resistance gene mutation and drug-resistance level in M. tuberculosis. Methods 108 M. tuberculosis clinical isolated strains from sputum specimens were analyzed by PCR-SSCP and traditional drug susceptibility tests. Results the gene mutation rate of SM, RFP, INH and EMBresistance climical isolated strains was 78.5%, 68.2%, 70.5% and 48.6% respectively, and the mutation rate of SM, RFP, INH and EMB high concentration resistance isolated strains was 86.5% , 89% , 84% and 48.6% respectively, but 28.5 % , 16.6% and 7.1% was the mutation rate of low concentration resistance strains. Conclusion The gene mutation was in relation with drug resistance level of M. tube rculosis. The gene mutation rate was hiher in high concentration resistance isolated strains than in low concentration resistane isolated strains.