| 黑曲霉B0201利用五倍子固体发酵产单宁酶的条件研究 |
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| 引用本文： | 李秧针,邱树毅,保玉心,吴鑫颖.黑曲霉B0201利用五倍子固体发酵产单宁酶的条件研究[J].微生物学通报,2009,36(8):1123-1129. |
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| 作者姓名： | 李秧针 邱树毅 保玉心 吴鑫颖 |
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| 作者单位： | 1. 贵州省发酵工程与生物制药重点实验室,贵州,贵阳,550003;贵州大学化工学院,贵州,贵阳,550003
2. 贵州省发酵工程与生物制药重点实验室,贵州,贵阳,550003;贵州大学生命科学学院,贵州,贵阳,550000 |
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| 基金项目： | 贵州省自然科学基金(No. J20082091) |
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| 摘 要： | 通过比较常规灭菌发酵和生料发酵, 研究黑曲霉B0201利用五倍子固体发酵产单宁酶的条件。结果表明, 在生料发酵过程中, 采用20%五倍子并且以(NH4)2SO4为氮源制备单宁酶的最佳条件为: 液固比=1.6:1、温度30°C、初始pH 6.0。在该条件下通过96 h的培养, 单宁酶的活力可达51.2 U/gds, 是常规灭菌发酵的3.6倍。以上结果显示, 生料发酵生产单宁酶是一种高效可行的方法。
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| 关 键 词： | 黑曲霉 单宁酶 生料发酵 五倍子 固体发酵 |
Research on Conditions of Tannase Production from Aspergillus Niger B0201 Under Solid-state Fermentation Using Gallnut |
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| LI Yang-Zhen,QIU Shu-Yi,BAO Yu-Xin and Wu Xin-Ying.Research on Conditions of Tannase Production from Aspergillus Niger B0201 Under Solid-state Fermentation Using Gallnut[J].Microbiology,2009,36(8):1123-1129. |
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| Authors: | LI Yang-Zhen QIU Shu-Yi BAO Yu-Xin and Wu Xin-Ying |
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| Institution: | Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guiyang, Guizhou 550003, China; Chemical Engineering College of Guizhou University, Guiyang, Guizhou 550003, China;Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guiyang, Guizhou 550003, China; Chemical Engineering College of Guizhou University, Guiyang, Guizhou 550003, China;Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guiyang, Guizhou 550003, China; Life Science College of Guizhou University, Guiyang, Guizhou 550000, China;Guizhou Province Key Laboratory of Fermentation Engineering and Biopharmacy, Guiyang, Guizhou 550003, China; Chemical Engineering College of Guizhou University, Guiyang, Guizhou 550003, China |
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| Abstract: | According to the comparative research of conventional sterile fermentation and uncooked material fermentation, the conditions of tannase production from Aspergillus Niger B0201 under solid-state fermentation using gallnut was studied. The results showed that the liquid?solid ratio of 1.6: 1, temperature of 30°C, initial pH of 6.0 were the optimal conditions for tannase production under uncooked material fermentation with gallnut content of 20% and (NH4)2SO4 for nitrogen sources. Under this condition, tannase activity reached to 51.2 U/gds after 96 h of cultivation, which was 3.6 times as the conventional sterile fermentation. The results above revealed that under uncooked material fermentation for tannase production was a feasible method with high efficiency. |
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| Keywords: | Aspergillus niger Tannase Uncooked material fermentation Gallnut Solid-state fermentation |
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