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| 转基因棉花根际土壤DNA的提取方法研究 | |
| 引用本文： | 李长林,吴建波,杨殿林,刘万华.转基因棉花根际土壤DNA的提取方法研究[J].微生物学通报,2007,34(5):0943-0945. |
| 作者姓名： | 李长林 吴建波 杨殿林 刘万华 |
| 作者单位： | 1. 农业部环境保护科研监测所,天津,300191 2. 南开大学生命科学学院,天津,300071 3. 武清区农业技术推广中心,天津,301700 |
| 基金项目： | 农业部环境保护科研监测所所长基金 |
| 摘 要： | 采用直接裂解方式,通过玻璃珠、溶菌酶和SDS共同作用直接从转基因棉花SGK321和中棉所41的根际土壤中提取微生物DNA。结果表明,该方法能从两种转基因棉花根际土壤中提取到20kb大小的完整的DNA片段。所得DNA完全适用于PCR扩增和酶切的要求。 |
| 关 键 词： | 转基因棉花 根际土壤微生物 |
| 文章编号： | 0253-2654（2007）05-0943-03 |
| 修稿时间： | 2006-12-18 |
Study on DNA Extraction Method in Rhizosphere Soil of Transgenic Cotton |
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| LI Chang-Lin,WU Jian-Bo,YANG Dian-Lin and LIU Wan-Hua.Study on DNA Extraction Method in Rhizosphere Soil of Transgenic Cotton[J].Microbiology,2007,34(5):0943-0945. | |
| Authors: | LI Chang-Lin WU Jian-Bo YANG Dian-Lin and LIU Wan-Hua |
| Institution: | 1 Agro-environmental Protection Institute, Ministry of Agriculture, Tianjin 300191;2 College of Life Science, Nankai University, Tianjin 300071;3 Agro-technlque Extension Center, Wuqing District, Tianjin 301700 |
| Abstract: | A direct lysis manner for extracting DNA directly from rhizosphere soil of transgenic cotton SGK321 and Zhongmiansuo 41 was designed by glass bead-lysozyme-SDS method. The results showed that intact DNA fragments over 20 kb were gained from rhizosphere soil planted two cultivars of transgenic cotton. DNA extracted suited to PCR amplification and RFLP. |
| Keywords: | RFLP |
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