CRISPR基因编辑技术虚拟仿真教学软件的构建及应用

CRISPR基因编辑技术逐渐成为一个强有力的分子技术,已越来越广泛地应用于科学研究和临床试验。在高校教学实践中,为了解决该实验原理复杂、操作难度大、周期长、成本高等问题,构建了CRISPR基因编辑仿真教学软件。该软件包括原理演示和实训操作2个模块。原理部分通过3D技术呈现出每个分子的结构,并以动画的形式演绎CRISPR-Cas9的分子机制。实训操作部分模拟了整个实验的操作过程,学生可以通过互动的形式在电脑上反复学习和操作。该交互式虚拟实验能激发学生的学习兴趣,显著提高实验教学质量。

Construction and application of virtual simulation teaching software of CRISPR gene editing technology

CRISPR,a gene editing technology,has become a powerful molecular technology which has been increasingly applied in scientific research and clinical trials.In order to solve the problems of high complexity in principle,difficulties in operation,high time consumption and economic cost,we have constructed a virtual simulation teaching software of CRISPR gene editing technology that can be utilized in the experimental teaching.The system consists of two modules,which are principle demonstration and operation training.In the principle part,the structure of each molecule is presented by 3D technology,and the molecular mechanism of CRISPR-Cas9 is displayed in the form of animation.The practical operation part simulates the operation process of the whole experiment,and students can learn and operate repeatedly on the computer in the form of interaction.This interactive virtual experiment can stimulate students’interest in learning,as well as significantly improving the quality of experimental teaching.