细菌解磷能力测定方法的研究

选择分解有机磷能力较强的3株细菌和溶解磷矿粉较强的4株细菌，砂培4周后，用不同的方法测定水浸提液中磷的含量。发现不同的细菌解磷能力差异很大，细菌在分解磷化合物的同时，一部分磷被细菌同化，一部分以无机磷酸盐状态贮藏在细菌细胞内。直接测定浸提液中无机磷酸盐的含量，将大大低估细菌的解磷能力，必须将浸提液消煮，才能比较正确地反映细菌分解磷的能力。

THE METHODS FOR QUANTIFYING CAPACITY OF BACTERIA IN DISSOLVING P COMPOUNDS

Three bacteria of decomposing lecithin and 4 bacteria of dissolving aptite were incubated for 4 weeks with sand media respectively. Phosphorus in the sand was extracted with distilled water and measured by different methods. It was found that the bacteria have a quite different ability to release P from the materials. Part of the P released became organic phosphorus compounds in microbial tissue. However, a large amount of the P was reserved in microbial cells in a form of phosphates. The direct measurement of P in the extract by molybdenum blue method would underestimate the capacity of the bacteria to release P from the materials. The correct approach was that the sand was fumigated with chloroform and then digested with acid before the measurement by molybdenum blue method.