海洋放线菌124092细胞毒活性和化学成分研究

采用MTT法对海洋放线菌124092正己烷提取物进行细胞毒活性筛选，结果显示对小鼠B16黑色素瘤细胞有一定的生长抑制活性。用硅胶真空柱层析法将正己烷提取物粗分为6个组分（Fr1～Fr6），细胞毒活性追踪显示Fr6组分为活性部分。为确定其中的活性成分，运用GC／MS对脯组分的化学成分进行了分析，结果显示其主要成分为：棕榈酸（Palmitic acid，11．76％）、油酸（Oleic acid，12．16％）、亚油酸（Linoleic acid．14．77％）和乳杆（菌）酸（Lactobacillic acid，61．31％）。据文献报道棕榈酸、油酸、亚油酸均对小鼠腹水瘤细胞具有细胞毒活性，亚油酸还对人肺腺癌细胞具有抑制作用。

Study on Cytotoxic Activity and Chemical Constitunents of Marine Actinomycets Strain 124092

The hexane extract from marine actinomycetes 124092 showed potent inhibition on B16 cell line by MTT assay. The hexane extract was fractionationed on silica gel column by vacuum liquid chromatography to afford 6 fractions(Fr1~Fr6), and Fr6 showed cytotoxic activity. To determine the bioacitve components of hexane extract, Fr6 was analyzed by GC/MS. The main components were identified as palmitic acid (11.76%), oleic acid (12.16%), linoleic acid (14.77%), and lactobacillic acid (61.31%). It have been reported that palmitic acid, oleic acid, and linoleic acid possess cytotoxic activity on rat ascites tumor cells and linoleic acid have suppressive effect on human lung adenocarcinoma cells.