一种寄生家蚕被毛孢的鉴定及产孢条件优化

【目的】对寄生家蚕的真菌被毛孢进行鉴定和孢子培养进行研究。【方法】采用形态特征比较和内转录间隔区(ITS)序列构建系统树进行鉴定,通过单因素筛选和正交试验进行产孢条件优化。【结果】根据形态特征比较和系统发育分析,该真菌为鹿儿岛被毛孢Hirsutella satumaensis Aoki。较优产孢条件为(质量体积比):蛋白胨3%,葡萄糖1%,蚕蛹粉1.5%,维生素B1 1%,硫酸镁0.05%,磷酸二氢钾0.1%,琼脂2%,蒸馏水1 000 mL,25°C。【结论】鹿儿岛被毛孢为已知种,文中对其显微特征进行了重新描述并补充相关分子系统学资料;产孢条件的优化可为该类群真菌孢子的获得与应用提供参考。

Identification of a parasitic Hirsutella Pat. of Bombyx mori Linnaeus. and sporulation conditions optimization

Objective] The objectives are to identify a parasitic Hirsutella Pat. of Bombyx mori Linnaeus. and to research the spore culture of this fungus. Methods] The comparison of morphological characteristics and internal transcribed spacer (ITS) sequence analysis were used for identification of specimens. single factor test and orthogonal test were carried out for studying sporulation conditions optimization. Results] According to the morphological characterization and phylogenetic tree, the fungus was identified as Hirsutella satumaensis Aoki. The optimum conditions for sporulation is: peptone 3%, glucose 1%, silkworm chrysalis powder 1.5%, vitamin B1 1%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1%, agar 2%, distilled water 1 000 ml, 25 °C. Conclusion] H. satumaensis Aoki, a known species in China, which microscopic characteristics were described again and the associated molecular systematics data were added in this paper. The sporulation conditions optimization could provide a reference for the spores acquisition and application of this group of fungi.