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天然介体在产漆酶真菌氧化蒽和芘中的重要作用(英文稿)
引用本文:李烜桢,王焰,吕正勇,王丰青,茹瑞红,高峰,钟卫鸿.天然介体在产漆酶真菌氧化蒽和芘中的重要作用(英文稿)[J].微生物学通报,2014,41(7):1293-1298.
作者姓名:李烜桢  王焰  吕正勇  王丰青  茹瑞红  高峰  钟卫鸿
作者单位:1. 河南农业大学 林学院 河南 郑州 450002;2. 浙江工业大学 生物与环境工程学院 浙江 杭州 310032;2. 浙江工业大学 生物与环境工程学院 浙江 杭州 310032;3. 北京高能时代环境技术股份有限公司 北京 100098;4. 河南农业大学 农学院 河南 郑州 450002;4. 河南农业大学 农学院 河南 郑州 450002;4. 河南农业大学 农学院 河南 郑州 450002;2. 浙江工业大学 生物与环境工程学院 浙江 杭州 310032
基金项目:Foundation by Key Laboratory of Soil Environment and Pollution Remediation; Institute of Soil Science, Chinese Academy of Sciences and Zhejiang Provincial Natural Science Foundation of China (no. Y5110147)
摘    要:【目的】研究了氧化还原介体在产漆酶真菌氧化蒽和芘的作用。【方法】通过非变性电泳和酶活力分析。【结果】发现血红密孔菌Z-1和木蹄层孔菌Z-5只产漆酶,其最大酶产量分别为11.90 U/mL和4.83 U/mL,不产木质素过氧化酶和锰过氧化物酶。木蹄层孔菌Z-5的胞外液尽管具有较低的漆酶活性,但是氧化了74.3%的蒽和12.4%的芘,高于血红密孔菌Z-1对蒽和芘的氧化率,提示天然介体可能存在于真菌胞外液中并且影响了漆酶对多环芳烃的氧化。实验进一步表明,木蹄层孔菌Z-5灭活和不灭活的超滤液以及灭活的胞外液对纯漆酶氧化多环芳烃的促进作用均大于血红密孔菌Z-1,说明木蹄层孔菌Z-5的天然介体比血红密孔菌Z-1能够更为有效地促进多环芳烃氧化。【结论】氧化还原结体在产漆酶真菌降解底物过程中发挥了重要作用,这也解释了木蹄层孔菌Z-5胞外液尽管漆酶活性不高,但是具有较大多环芳烃氧化率的原因。

关 键 词:漆酶,多环芳烃,白腐真菌,氧化

Important role of natural mediators in oxidation of anthracene and pyrene by laccase producing fungi
Institution:1. College of Forest, Henan Agricultural University, Zhengzhou, Henan 450002, China; 2. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310032, China;2. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310032, China;3. Beijing GeoEnviron Engineering & Technology, Inc., Beijing, 100098, China;4. Agronomy College, Henan Agricultural University, Zhengzhou, Henan 450002, China;4. Agronomy College, Henan Agricultural University, Zhengzhou, Henan 450002, China;4. Agronomy College, Henan Agricultural University, Zhengzhou, Henan 450002, China;2. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, Zhejiang 310032, China
Abstract:Objective] The role of natural mediators in oxidation of anthracene and pyrene by laccase producing fungi were evaluated. Methods] In this work, enzyme activities assay and nondenaturing PAGE were employed to analyze laccase activity. Results] The results showed that the fungus Pycnoporus sanguineus Z-1 and Fomes fomentarius Z-5 produced laccase, with maximal production of 11.90 and 4.83 U/mL, respectively, but not lignin peroxidase and manganese peroxidase. However, the culture fluid of Fomes fomentarius Z-5, with lower laccase activity, oxidized 74.3% of anthracene and 12.4% of pyrene, higher than that of Pycnoporus sanguineus Z-1, which suggested that the natural mediators might exist in the fungal culture and influenced the anthracene and pyrene oxidation. A further experiment demonstrated that all the treatments with addition of ultrafiltrate, boiled ultrafiltrate or boiled culture fluid improved the antracene and pyrene oxidation. The enhancement levels of ultrafiltrate, boiled ultrafiltrate and boiled culture fluid from Fomes fomentarius Z-5 were higher than those of Pycnoporus sanguineus Z-1, implying that the natural mediators in Fomes fomentarius Z-5 culture was more efficient in improving PAHs oxidation than in Pycnoporus sanguineus Z-1 culture. Conclusion] The findings indicated that natural mediators played a important role in oxidation of substrates by laccase producing fungi and these might account for the phenomenon that Fomes fomentarius Z-5 culture, with lower laccase activity, oxidized more anthracene and pyrene than Pycnoporus sanguineus Z-1 culture.
Keywords:Laccase  Polycyclic aromatic hydrocarbons  White rot fungi  Oxidation
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