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假单胞菌荧光与非荧光铁载体对铁离子的应答差异
引用本文:陈绍兴,费宗伟,何明涛,沈 萍,谢志雄.假单胞菌荧光与非荧光铁载体对铁离子的应答差异[J].微生物学通报,2008,35(10):1572-1576.
作者姓名:陈绍兴  费宗伟  何明涛  沈 萍  谢志雄
作者单位:1. 红河学院,生命科学与技术学院,蒙自,661100;武汉大学,生命科学学院,武汉,430072
2. 红河学院,生命科学与技术学院,蒙自,661100
3. 武汉大学,生命科学学院,武汉,430072
基金项目:武汉市科学与技术晨光计划项目
摘    要:假单胞菌既能产荧光铁载体也能产非荧光铁载体.通过对假单胞菌在不同铁离子浓度下,在通用CAS(Chrome azroul S)检测平板、改进的蔗糖-天冬氨酸(SA)平板(MSA)上以及通用液体CAS培养基和MSA培养基内的铁载体产生情况的比较,发现在通用CAS的液体培养基上产生的主要为非荧光铁载体(pyochelin),而在改进的MSA培养基上产生的主要为荧光铁载体(pyoverdine);在铁离子的应答方面,pyoverdine较pyochelin灵敏,较低的铁离子浓度即可抑制荧光铁载体的产生,但是不能抑制非荧光铁载体.

关 键 词:铁载体  铁离子  假单胞菌

Different Response of Dluorescent and Non-fluorescent Siderophores from Pseudomonas to Ferric Iron
CHEN Shao-Xing,FEI Zong-Wei,HE Ming-Tao,SHEN Ping and XIE Zhi-Xiong.Different Response of Dluorescent and Non-fluorescent Siderophores from Pseudomonas to Ferric Iron[J].Microbiology,2008,35(10):1572-1576.
Authors:CHEN Shao-Xing  FEI Zong-Wei  HE Ming-Tao  SHEN Ping and XIE Zhi-Xiong
Institution:(1. College of Life Sciences and Technology, Honghe University, Mengzi 661100) (2. College of Life Sciences, Wuhan University, Wuhan 430072);College of Life Sciences and Technology, Honghe University, Mengzi 661100;College of Life Sciences and Technology, Honghe University, Mengzi 661100;College of Life Sciences, Wuhan University, Wuhan 430072;College of Life Sciences, Wuhan University, Wuhan 430072
Abstract:We have studied the influence of ferric iron on the production of siderophore (microbial iron transport compound) from Pseudomonas sp. on universal CAS (Chrome azroul S) assay agar and modified sugar-aspartic acid (MSA) agar or liquid medium respectively. The results showed that the non-fluorescent siderophores (pyochelin) were produced on universal CAS assay agar and liquid medium and fluorescent siderophores (pyoverdine) on MSA agar and liquid medium; and the pyoverdine was more sensitive to the response of ferric iron whatever on agar plate or in liquid medium.
Keywords:Pyochelin  Pyoverdine
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