樟绒枝霉(Malbranchea cinnamomea)固体发酵产木聚糖酶的发酵条件优化

目的] 研究樟绒枝霉(Malbranchea cinnamomea) CAU521利用农业废弃物固体发酵产木聚糖酶的发酵条件.方法]采用单因素试验法优化影响菌株产酶的各个条件,包括碳源种类、氮源种类、初始pH、初始水分含量、培养温度及发酵时间共6个因素.结果]获得的最佳产酶条件为:稻草为发酵碳源、2％(W/W)的酵母提取物为氮源、初始pH 7.0、初始水分含量80％和发酵温度45℃.在此条件下发酵6d后木聚糖酶的酶活力达到13 120 U/g干基碳源.结论]樟绒枝霉固体发酵产木聚糖酶的产酶水平高,生产成本低,具有潜在的工业化应用前景.

Optimization of xylanase production from Malbranchea cinnamomea by solid state fermentation

Objective] We optimized xylanase production from Malbranchea cinnamomea CAU521 by solid state fermentation (SSF) with agricultural wastes as carbon source. Methods] single-factor experiment was used to optimized various factors affecting the enzyme production. There are six factors which include carbon source, nitrogen source, initial pH, initial moisture content, temperature and fermentation time were investigated. Results] the optimum conditions for xylanase production were as follows: rice straw as carbon source, 2%?(w/w) yeast extract as nitrogen source, the ratio of solid substrate to mineral solution of 1:4 (W/V), the initial pH of 7.0, and incubation temperature of 45 °C. Under the optimized conditions, the highest xylanase production of 13 120 U/g dry carbon source was achieved after 6 days of cultivation. Conclusion] the high-level xylanase production and low production cost may facilitate industrial scale enzyme application.