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PNP降解相关基因温敏突变株的分离及其特性研究
引用本文:邓海华,李晓丹,曹慧,王世明,黄婷婷,崔中利.PNP降解相关基因温敏突变株的分离及其特性研究[J].微生物学通报,2007,34(1):0032-0035.
作者姓名:邓海华  李晓丹  曹慧  王世明  黄婷婷  崔中利
作者单位:南京农业大学生命科学学院微生物学系,农业部农业环境微生物工程重点开放实验室,南京,210095
基金项目:国家高技术研究发展计划(863计划);国家自然科学基金
摘    要:通过接合转移将质粒pSC123上的转座子Tn5随机插入到DLL-E4基因组DNA中,从大约8,000个突变株中得到1株温度敏感型突变株MT54。根据转座子上的已知序列设计引物以MT54基因组DNA为模板进行PCR扩增,证实MT54的染色体中有转座子插入。MT54在30℃条件下能够以对硝基苯酚(p-nitrophenol,PNP)为唯一碳源生长,但在37℃不能生长。格里斯试色剂法检测NO_2~-的生成情况进一步证明了MT54的这种特性。通过30℃和37℃两种温度条件下MT54和原始出发菌株DLL-E4对PNP和对苯二酚降解情况的比较,推测温敏突变位点可能发生在与PNP降解相关的基因中。

关 键 词:对硝基苯酚  对苯二酚  转座子突变  温敏突变株
文章编号:0253-2654(2007)01-0032-04
修稿时间:2006-03-09

Isolation and Characterization of a Temperature-sensitive p-Nitrophenol Degradation Related Genes Mutant Strain
DENG Hai-Hu,LI Xiao-Dan,CAO Hui,WANG Shi-Ming,HUANG Ting-Ting and CUI Zhong-Li.Isolation and Characterization of a Temperature-sensitive p-Nitrophenol Degradation Related Genes Mutant Strain[J].Microbiology,2007,34(1):0032-0035.
Authors:DENG Hai-Hu  LI Xiao-Dan  CAO Hui  WANG Shi-Ming  HUANG Ting-Ting and CUI Zhong-Li
Institution:Key Lab of Microbiology Engineering of Agricultural Environment; Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095;Key Lab of Microbiology Engineering of Agricultural Environment; Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095;Key Lab of Microbiology Engineering of Agricultural Environment; Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095;Key Lab of Microbiology Engineering of Agricultural Environment; Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095;Key Lab of Microbiology Engineering of Agricultural Environment; Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095;Key Lab of Microbiology Engineering of Agricultural Environment; Ministry of Agriculture; Nanjing Agricultural University; Nanjing 210095
Abstract:
Keywords:p-Nitrophenol  Hydroquinone  Transposon insertion mutant  Temperature-sensitive mutant
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