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利用响应面法优化ε-聚赖氨酸发酵培养基
引用本文:张超,张克峰,侯书国,马永山.利用响应面法优化ε-聚赖氨酸发酵培养基[J].微生物学通报,2011,38(5):641-646.
作者姓名:张超  张克峰  侯书国  马永山
作者单位:山东建筑大学市政与环境工程学院,山东,济南,250101
摘    要:采用响应面法对白色链霉菌ZC7发酵合成ε-聚赖氨酸的培养基进行优化研究。采用Plackett-Burman法对8个因素进行了筛选,结果表明,葡萄糖、酵母膏和硫酸铵的浓度对ε-聚赖氨酸产量影响较大。用最陡爬坡试验及Box-Behnken设计进一步优化,利用Design-Expert软件进行二次回归分析,得到各因素的最佳浓度为:葡萄糖37.22 g/L,酵母膏6.9 g/L,硫酸铵6.55 g/L。在此优化条件下ε-聚赖氨酸的产量达到8.11 g/L,较单因素试验最高值提高24.6%。

关 键 词:白色链霉菌  ε-聚赖氨酸  优化  响应面

Medium optimization of ε-polylysine production by response surface analysis
ZHANG Chao,ZHANG Ke-Feng,HOU Shu-Guo and MA Yong-Shan.Medium optimization of ε-polylysine production by response surface analysis[J].Microbiology,2011,38(5):641-646.
Authors:ZHANG Chao  ZHANG Ke-Feng  HOU Shu-Guo and MA Yong-Shan
Institution:School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, Shandong 250101, China
Abstract:In order to enhance the concentration of ε-polylysine(ε-PL) with Streptomyces albulus ZC7,response surface methodology were used to optimize the fermentation medium.A Plackett-Burman de-sign was used to evaluate the influence of eight factors firstly.Results showed that the concentration of glucose,yeast extract and(NH4)2SO4 played important roles in influencing the content of ε-PL.Then,the path of steepest ascent and the Box-Behnken design were adopted for further optimization,and the optimum concentration levels and the relationships among these factors was found out by quadratic re-gression model equation with Design-Expert statistic methods,the optimal concentration of the vari-ables were determined as: glucose 37.22 g/L,yeast extract 6.9 g/L,(NH4)2SO4 6.55 g/L.Under such conditions,the concentration of ε-PL was increased to 8.11 g/L,which was 24.6% higher than the maximum value in the single factor tests.
Keywords:Streptomyces albulus  ε-poly-L-lysine  Optimization  Response surface methodology
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